Detailed information of TA system TA065420

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2434..3085
• Replicon: plasmid pGFJ4
• Accession: NZ_CP016900
• Organism: Acinetobacter soli strain GFJ2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: BEN76_RS17155
• Protein ID: WP_055414415.1
• Coordinates: 2434..2790 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BEN76_RS17160
• Protein ID: WP_055414414.1
• Coordinates: 2783..3085 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BEN76_RS17150	951..1922	+	972	WP_005176780.1	helix-turn-helix domain-containing protein	-
BEN76_RS17155	2434..2790	+	357	WP_055414415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BEN76_RS17160	2783..3085	+	303	WP_055414414.1	XRE family transcriptional regulator	Antitoxin
BEN76_RS17165	3285..3803	+	519	WP_076033782.1	sel1 repeat family protein	-
BEN76_RS17170	4007..4564	-	558	WP_076033783.1	plasmid replication DNA-binding protein	-
BEN76_RS17175	4628..5551	-	924	WP_055414407.1	replication initiation protein RepM	-
BEN76_RS17180	5801..5995	-	195	WP_076033784.1	hypothetical protein	-
BEN76_RS17185	6240..6962	-	723	WP_076033785.1	hypothetical protein	-
BEN76_RS17190	7174..8064	+	891	WP_076033786.1	MobA/MobL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..9416	9416

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13559.50 Da
• Isoelectric Point: 5.7286

>T65420 WP_055414415.1 NZ_CP016900:2434-2790 [Acinetobacter soli]
MWTVITTDLFNEWLEQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYNSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIADKGGKKRFYKEMLDIADEQYELHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T65420 NZ_CP016900:2434-2790 [Acinetobacter soli]
ATGTGGACCGTCATCACGACAGATCTGTTTAATGAATGGCTTGAACAGCAAGACGAATCAACACAAGAGAAAGTCCTAGC
CGCTTTGGTTGTTCTACAGCAGCAGGGACCAAGTTTAGGTCGCCCTTTAGTGGATACCGTGTATAACTCTAAATTTACCA
ATATGAAAGAGCTACGTGTTCAGCATCGAGGTAAACCACTGAGAGCCTTTTTCGCATTTGATCCATTACGACAAGCCATT
GTGCTGTGCATTGCTGATAAAGGCGGAAAAAAGCGATTTTATAAAGAGATGCTGGATATTGCAGATGAGCAATACGAACT
TCATCTCTCAACCCTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11214.02 Da
• Isoelectric Point: 6.4759

>AT65420 WP_055414414.1 NZ_CP016900:2783-3085 [Acinetobacter soli]
MAKTLQELLASRSPESQARIQKMADELLLETQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT65420 NZ_CP016900:2783-3085 [Acinetobacter soli]
ATGGCTAAAACATTACAGGAATTATTGGCTAGTCGCTCTCCAGAGAGCCAAGCTCGTATTCAAAAAATGGCTGATGAACT
ACTTTTAGAAACTCAGCTTCACCTTATTCGAGAAGAATTAGAGATTTCCCAAAAAGAACTGGCTGAAACTCTAGGAATCA
AACAACCATCACTTTCAGCAATCGAAAACCGTGGCAATGACCTTAAGATTTCGACCATGAAAAAATACGTTGAAGCGATG
GGGGGAAAGCTCCGTATTGATGTAGAGCTTCCAACAGGAAAGCATATAGGATTCAATGTGTAA