Detailed information of TA system TA065418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 23478..24171
• Replicon: plasmid pGFJ1
• Accession: NZ_CP016897
• Organism: Acinetobacter soli strain GFJ2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: R9B971
• Locus tag: BEN76_RS16295
• Protein ID: WP_016165531.1
• Coordinates: 23478..23768 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BEN76_RS16300
• Protein ID: WP_076033629.1
• Coordinates: 23770..24171 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BEN76_RS16295	23478..23768	+	291	WP_016165531.1	type II toxin-antitoxin system HigB family toxin	Toxin
BEN76_RS16300	23770..24171	+	402	WP_076033629.1	helix-turn-helix domain-containing protein	Antitoxin
BEN76_RS16305	24487..24675	+	189	WP_076033630.1	type II toxin-antitoxin system HicA family toxin	-
BEN76_RS16310	24678..25064	+	387	WP_076033631.1	type II toxin-antitoxin system HicB family antitoxin	-
BEN76_RS16315	25439..25653	-	215	Protein_16	hypothetical protein	-
BEN76_RS16320	25725..26546	-	822	WP_076033632.1	HNH endonuclease	-
BEN76_RS16325	26605..26799	-	195	WP_076033633.1	exodeoxyribonuclease VII small subunit	-
BEN76_RS16330	26774..28054	-	1281	WP_076033634.1	exodeoxyribonuclease VII large subunit	-
BEN76_RS16335	28690..29040	+	351	WP_076033635.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..86373	86373

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (19-90)

Antitoxin

• Cro/C1-type helix-turn-helix domain (79-132)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11257.12 Da
• Isoelectric Point: 10.1259

>T65418 WP_016165531.1 NZ_CP016897:23478-23768 [Acinetobacter soli]
MHVITHARIIEAMHKWPQAETALDGWYRTIKANDPKDFAEMKQLFPAVDKVGKFHVFDIGGNKIRLIAVVMYQAKRVYIR
HVLSHKEYDKGHWKEG

Nucleotide

• Length: 291 bp

>T65418 NZ_CP016897:23478-23768 [Acinetobacter soli]
ATGCATGTGATCACACACGCTCGAATTATAGAAGCAATGCACAAGTGGCCACAGGCTGAAACGGCATTAGATGGTTGGTA
TCGAACCATAAAGGCAAACGATCCGAAAGATTTTGCTGAGATGAAGCAGCTCTTTCCAGCGGTCGATAAAGTTGGAAAGT
TCCATGTATTCGATATTGGCGGGAACAAGATCCGACTTATTGCAGTAGTGATGTATCAGGCAAAACGTGTGTATATCCGG
CATGTCTTGTCCCATAAAGAATATGACAAAGGTCATTGGAAGGAAGGTTAG

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14816.67 Da
• Isoelectric Point: 4.5307

>AT65418 WP_076033629.1 NZ_CP016897:23770-24171 [Acinetobacter soli]
MNAMIKQAVDHWHYVAPLLSRPANEDDFHTLVEALDELLDTVGDDESHPLMGLIHQLGDLVSDYENEHLPMPKGDGRAAL
AFLMEQHGLGQSDLAEVATQSVISEILSGKRQLNIRHIKALSERFNVSADTFF

Nucleotide

• Length: 402 bp

>AT65418 NZ_CP016897:23770-24171 [Acinetobacter soli]
ATGAACGCGATGATTAAACAGGCCGTAGACCACTGGCACTACGTTGCACCATTGCTCAGCAGGCCAGCAAATGAAGACGA
CTTTCATACACTTGTTGAAGCTTTAGATGAACTTTTAGATACCGTTGGTGATGATGAGTCTCATCCTTTAATGGGGCTTA
TTCATCAGCTTGGTGATTTGGTTTCTGATTATGAAAATGAGCATCTTCCTATGCCTAAAGGGGATGGTCGCGCTGCTTTG
GCTTTTTTGATGGAGCAACATGGTTTAGGTCAGTCTGACTTGGCAGAGGTTGCAACCCAATCTGTGATTTCTGAAATTTT
AAGTGGTAAACGCCAACTGAATATTCGTCATATTAAGGCACTATCTGAGCGGTTTAATGTATCGGCAGATACGTTTTTCT
AA

Structures

Toxin

• AlphaFold DB: R9B971