Detailed information of TA system TA065401

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 404876..405527
• Replicon: plasmid unnamed1
• Accession: NZ_CP016890
• Organism: Pantoea agglomerans strain C410P1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: BEE12_RS21150
• Protein ID: WP_069026837.1
• Coordinates: 405183..405527 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BEE12_RS21145
• Protein ID: WP_069026836.1
• Coordinates: 404876..405190 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BEE12_RS21120	400778..401080	-	303	WP_069026834.1	type 1 fimbrial protein	-
BEE12_RS21125	401569..401988	-	420	WP_031594127.1	VOC family protein	-
BEE12_RS21130	402027..402908	-	882	WP_031594128.1	LysR family transcriptional regulator	-
BEE12_RS21135	403054..403905	+	852	WP_069026835.1	MBL fold metallo-hydrolase	-
BEE12_RS21140	403996..404666	-	671	Protein_367	hypothetical protein	-
BEE12_RS21145	404876..405190	-	315	WP_069026836.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
BEE12_RS21150	405183..405527	-	345	WP_069026837.1	toxin	Toxin
BEE12_RS21155	405617..406528	-	912	WP_069026838.1	LysR family transcriptional regulator	-
BEE12_RS21160	406626..407684	+	1059	WP_069026944.1	SDR family oxidoreductase	-
BEE12_RS21165	407742..408620	-	879	WP_033768811.1	23S rRNA pseudouridine(2604) synthase RluF	-
BEE12_RS21170	408724..409194	-	471	WP_031594134.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iroN	1..543504	543504

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Antitoxin MqsA/HigA-2 (18-100)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13486.26 Da
• Isoelectric Point: 9.6381

>T65401 WP_069026837.1 NZ_CP016890:c405527-405183 [Pantoea agglomerans]
MDALFIELSSFQKYRADYLNDDQYRLFQNMLLADPEKGDVIPDTGGLRKVRFRDERRHKGARGGIRVIYYWTNTQGQFIL
FTIYDKDQRDDLTRQQRDALATALNVIKKGLRHD

Nucleotide

• Length: 345 bp

>T65401 NZ_CP016890:c405527-405183 [Pantoea agglomerans]
ATGGACGCACTATTCATCGAACTTTCCAGCTTCCAGAAATACCGGGCTGATTATCTGAATGATGATCAGTACAGGCTGTT
TCAGAACATGTTGCTGGCTGACCCTGAGAAGGGCGATGTGATACCCGATACCGGCGGACTGCGTAAAGTTCGCTTCAGGG
ATGAGCGCCGACACAAGGGCGCACGTGGCGGGATCAGGGTTATCTATTACTGGACCAACACACAAGGCCAATTCATCCTG
TTTACCATCTACGATAAAGATCAACGCGACGATTTGACCAGGCAACAGCGTGATGCCCTGGCGACCGCGCTGAATGTGAT
CAAAAAAGGACTGAGACATGACTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12022.84 Da
• Isoelectric Point: 9.8806

>AT65401 WP_069026836.1 NZ_CP016890:c405190-404876 [Pantoea agglomerans]
MTERDIFSELMTGMQELKDHQEGKITLKSYKVTKRAPVTIAPQELRDVREKLNLSQAVFAHYLHTGETTYQNWEQGRARP
NAQAVLLIRMVQQNPETLKTLAKL

Nucleotide

• Length: 315 bp

>AT65401 NZ_CP016890:c405190-404876 [Pantoea agglomerans]
ATGACTGAACGCGATATCTTCAGTGAACTGATGACGGGTATGCAGGAGCTTAAGGATCATCAGGAGGGCAAAATTACGCT
TAAGAGTTATAAGGTCACTAAGCGTGCCCCTGTAACTATTGCGCCGCAGGAGTTACGCGACGTACGCGAAAAACTGAACC
TCTCACAGGCCGTATTCGCCCATTATCTTCATACTGGCGAAACGACATATCAAAACTGGGAACAGGGCAGGGCGAGACCT
AACGCCCAGGCTGTTTTACTGATCCGCATGGTGCAGCAGAACCCAGAAACGCTGAAGACGCTCGCGAAGCTCTGA