Detailed information of TA system TA065394

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 31658..32318
• Replicon: chromosome
• Accession: NZ_CP016889
• Organism: Pantoea agglomerans strain C410P1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: BEE12_RS00140
• Protein ID: WP_069025145.1
• Coordinates: 31965..32318 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BEE12_RS00135
• Protein ID: WP_010247918.1
• Coordinates: 31658..31960 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BEE12_RS00115	27497..28522	-	1026	WP_208863854.1	ABC transporter permease	-
BEE12_RS00120	28546..30030	-	1485	WP_069025144.1	sugar ABC transporter ATP-binding protein	-
BEE12_RS00125	30063..30986	-	924	WP_010247907.1	sugar ABC transporter substrate-binding protein	-
BEE12_RS00135	31658..31960	-	303	WP_010247918.1	XRE family transcriptional regulator	Antitoxin
BEE12_RS00140	31965..32318	-	354	WP_069025145.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BEE12_RS00145	32617..33705	-	1089	WP_029519619.1	hypothetical protein	-
BEE12_RS00150	34101..36263	+	2163	WP_081325906.1	ferric-rhodotorulic acid/ferric-coprogen receptor FhuE	-
BEE12_RS00155	36391..36663	-	273	WP_069025146.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (18-89)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13677.77 Da
• Isoelectric Point: 9.1083

>T65394 WP_069025145.1 NZ_CP016889:c32318-31965 [Pantoea agglomerans]
VWMIKTTERFDRWFTLLNESDRACVLAALMVLREKGPGLSRPYADTLKGSVYINMKELRIQSRGDPIRAFFAFDPNRTAI
VLCAGNKVGNEKRFYREMLPVADREFTHWLNSFKDEE

Nucleotide

• Length: 354 bp

>T65394 NZ_CP016889:c32318-31965 [Pantoea agglomerans]
GTGTGGATGATTAAAACGACGGAGAGGTTTGATCGCTGGTTTACCTTGCTTAATGAGAGCGATCGCGCCTGCGTACTGGC
AGCATTAATGGTGTTGCGCGAAAAAGGGCCGGGGTTGTCCAGGCCATACGCGGATACGTTAAAAGGCTCTGTTTATATCA
ACATGAAAGAGCTGCGTATTCAGAGTCGCGGTGATCCGATAAGGGCTTTCTTTGCTTTTGACCCAAACCGAACGGCGATT
GTGCTTTGTGCCGGAAATAAGGTGGGCAATGAAAAGCGTTTTTATCGGGAGATGCTTCCGGTTGCCGATCGGGAATTTAC
ACACTGGCTGAATAGTTTTAAAGATGAGGAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10941.72 Da
• Isoelectric Point: 5.7251

>AT65394 WP_010247918.1 NZ_CP016889:c31960-31658 [Pantoea agglomerans]
MGRTLEQLIADEKPEVVADAQAMATDILLNIHLAELREKVQKTQVEMAKALGITQPTVAGMEKPGRDLKLSTLKRYIDAA
GGKLRLVVELPDGSHYGFEV

Nucleotide

• Length: 303 bp

>AT65394 NZ_CP016889:c31960-31658 [Pantoea agglomerans]
ATGGGCAGAACGCTGGAACAGCTTATTGCGGATGAAAAACCGGAAGTCGTTGCCGACGCACAGGCGATGGCGACGGATAT
CCTGCTCAACATTCACCTTGCCGAGCTGCGTGAGAAAGTGCAGAAAACACAGGTCGAAATGGCAAAGGCGCTGGGCATTA
CACAGCCTACCGTGGCGGGCATGGAGAAGCCGGGCCGCGACCTGAAGCTCTCCACCCTCAAGCGCTATATCGATGCGGCA
GGGGGTAAGCTGCGGCTGGTGGTGGAACTGCCGGACGGCTCGCACTACGGGTTTGAGGTGTAA