65381

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 2974589..2975237 Replicon chromosome
Accession NZ_CP016888
Organism Mycobacterium tuberculosis strain SCAID 252.0

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag BEE64_RS14030 Protein ID WP_003899414.1
Coordinates 2974589..2974912 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag BEE64_RS14035 Protein ID WP_003899415.1
Coordinates 2974992..2975237 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BEE64_RS14005 2970160..2971421 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
BEE64_RS22235 2971448..2971636 - 189 WP_068986962.1 hypothetical protein -
BEE64_RS14015 2971798..2973237 - 1440 WP_003901443.1 phage major capsid protein -
BEE64_RS14020 2973245..2973778 - 534 WP_003899412.1 HK97 family phage prohead protease -
BEE64_RS21650 2973931..2974422 - 492 WP_003900541.1 phage terminase small subunit P27 family -
BEE64_RS14030 2974589..2974912 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
BEE64_RS14035 2974992..2975237 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
BEE64_RS14040 2975234..2976661 - 1428 WP_003899416.1 DUF3631 domain-containing protein -
BEE64_RS14045 2976663..2977055 - 393 WP_003899417.1 DUF2742 domain-containing protein -
BEE64_RS14050 2977052..2977312 - 261 WP_003899418.1 helix-turn-helix domain-containing protein -
BEE64_RS14055 2977329..2977691 - 363 WP_003900543.1 hypothetical protein -
BEE64_RS14060 2977694..2978821 - 1128 WP_003899420.1 site-specific integrase -
BEE64_RS14065 2978966..2979193 - 228 WP_003899421.1 hypothetical protein -
BEE64_RS21655 2979190..2979579 - 390 WP_003899422.1 hypothetical protein -
BEE64_RS14070 2979485..2979757 + 273 WP_003900544.1 hypothetical protein -
BEE64_RS14075 2979856..2980089 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2968551..2978821 10270


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Flavinator of succinate dehydrogenase

(10-82)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T65381 WP_003899414.1 NZ_CP016888:c2974912-2974589 [Mycobacterium tuberculosis]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T65381 NZ_CP016888:c2974912-2974589 [Mycobacterium tuberculosis]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT65381 WP_003899415.1 NZ_CP016888:c2975237-2974992 [Mycobacterium tuberculosis]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT65381 NZ_CP016888:c2975237-2974992 [Mycobacterium tuberculosis]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References