65327

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) HipBST/HipA(toxin)
Location 711973..713216 Replicon chromosome
Accession NZ_CP016876
Organism Legionella pneumophila subsp. pneumophila strain FFI337

Toxin (Protein)


Gene name HipT Uniprot ID Q5WU78
Locus tag BE846_RS03100 Protein ID WP_011216259.1
Coordinates 712278..713216 (+) Length 313 a.a.

Antitoxin (Protein)


Gene name HipS Uniprot ID Q5WU79
Locus tag BE846_RS03095 Protein ID WP_011216258.1
Coordinates 711973..712281 (+) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE846_RS03065 707114..708301 - 1188 Protein_589 transposase -
BE846_RS03070 708354..708788 - 435 Protein_590 hypothetical protein -
BE846_RS03080 709101..710612 + 1512 WP_129827326.1 Fic family protein -
BE846_RS03085 710651..711319 + 669 WP_011216256.1 hypothetical protein -
BE846_RS03090 711749..711973 + 225 WP_011216257.1 helix-turn-helix transcriptional regulator -
BE846_RS03095 711973..712281 + 309 WP_011216258.1 HipA N-terminal domain-containing protein Antitoxin
BE846_RS03100 712278..713216 + 939 WP_011216259.1 HipA domain-containing protein Toxin
BE846_RS03105 713741..714829 - 1089 WP_011216260.1 DDE-type integrase/transposase/recombinase -
BE846_RS03110 715185..716558 + 1374 WP_011216261.1 ATP-binding protein -
BE846_RS03115 716757..717662 + 906 WP_011216262.1 restriction endonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 717906..718307 401


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(53-275)

Antitoxin

HipA, N-terminal subdomain 1

(6-98)


Sequences

Toxin        


Download         Length: 313 a.a.        Molecular weight: 36016.80 Da        Isoelectric Point: 9.5712

>T65327 WP_011216259.1 NZ_CP016876:712278-713216 [Legionella pneumophila subsp. pneumophila]
MKRCPITYDLISDQENYSQRGLHLLSPQLKNLSPLDLSADEQRQEAIARVGKMSIQGVQKKLSAKLKIKEGGFEIVDQKG
QYILKPQSDIYPELPENEAITMTLAKAIGLEVPVHGLVYSKDNSLTYFIKRFDRIGHNKKLALEDFAQLSGEDRHTKYKS
SMEKVIAVIQQFCTFPKIEFVKLFKLTLFNFLVGNEDMHLKNFSLITKDRKISISPAYDLINSTIAQKNTKEEIALPLKG
KKNNLTKSDFLKYFAVEKLGLNQNVIDGIVQEFHQIIPEWRKLIGFSFLSQQMQEKYIELLDQRCKRFNFFD

Download         Length: 939 bp

>T65327 NZ_CP016876:712278-713216 [Legionella pneumophila subsp. pneumophila]
ATGAAACGCTGCCCTATTACTTATGACTTAATTAGTGACCAAGAAAACTATTCTCAACGTGGATTGCATTTACTTTCCCC
TCAATTAAAAAACCTTAGCCCATTAGATTTAAGTGCTGATGAGCAGCGACAGGAAGCAATTGCTCGTGTAGGAAAGATGT
CTATTCAAGGCGTCCAAAAGAAACTAAGTGCCAAACTAAAGATTAAGGAGGGAGGTTTTGAAATCGTTGATCAAAAGGGG
CAGTACATTTTAAAACCACAAAGTGATATTTACCCAGAATTGCCGGAAAATGAAGCCATTACTATGACCCTTGCAAAGGC
CATTGGCCTCGAAGTTCCGGTTCATGGTTTGGTTTATTCTAAAGACAACAGTTTGACCTACTTCATTAAACGCTTTGATA
GAATAGGCCATAATAAAAAGTTAGCTTTAGAAGATTTTGCACAGCTATCAGGTGAAGATCGACATACGAAATATAAAAGT
TCTATGGAAAAGGTAATTGCAGTCATACAGCAGTTTTGTACATTCCCAAAAATTGAATTCGTGAAATTATTTAAGCTGAC
GTTGTTTAACTTCCTGGTCGGCAATGAAGACATGCATCTAAAAAATTTTTCCTTAATTACTAAGGATAGAAAAATTTCCA
TATCACCAGCATATGATTTAATTAACTCAACTATCGCTCAAAAAAACACAAAAGAAGAAATCGCTTTGCCTCTGAAAGGG
AAAAAAAATAATTTAACAAAGAGTGATTTTCTTAAATACTTTGCCGTTGAAAAATTAGGATTAAATCAGAACGTCATCGA
TGGGATCGTACAAGAGTTCCATCAAATAATACCTGAATGGCGAAAACTTATTGGTTTCAGTTTCTTATCTCAACAGATGC
AGGAAAAATATATCGAATTGCTCGATCAGCGATGCAAGCGATTCAATTTTTTTGATTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11654.54 Da        Isoelectric Point: 4.6017

>AT65327 WP_011216258.1 NZ_CP016876:711973-712281 [Legionella pneumophila subsp. pneumophila]
MRKAYISVNGIRAGILEELEGKKYQFTYLNDYQGAPVSLTMPLTNKIYDFDVFPPFFEGLLPEGIMLEALLRKYKIDKND
YFGQLIIVGQDVVGAVTIEELR

Download         Length: 309 bp

>AT65327 NZ_CP016876:711973-712281 [Legionella pneumophila subsp. pneumophila]
ATGAGAAAAGCATACATATCAGTGAATGGCATTAGAGCTGGAATATTAGAGGAGTTAGAAGGTAAAAAATATCAATTTAC
TTACCTTAATGATTATCAGGGAGCACCTGTTTCTCTCACCATGCCGTTAACCAATAAGATCTATGATTTTGACGTATTTC
CCCCTTTTTTTGAAGGATTGCTACCTGAAGGTATTATGCTTGAAGCATTATTACGCAAATATAAAATCGATAAAAATGAT
TATTTTGGTCAATTGATAATAGTTGGCCAAGATGTCGTAGGTGCAGTGACGATTGAGGAACTAAGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q5WU78

Antitoxin

Source ID Structure
AlphaFold DB A0A317U6X7

References