65324

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) HipBST/HipA(toxin)
Location 2576327..2577570 Replicon chromosome
Accession NZ_CP016874
Organism Legionella pneumophila subsp. pneumophila strain FFI329

Toxin (Protein)


Gene name HipT Uniprot ID Q5WU78
Locus tag BE845_RS11520 Protein ID WP_011216259.1
Coordinates 2576327..2577265 (-) Length 313 a.a.

Antitoxin (Protein)


Gene name HipS Uniprot ID Q5WU79
Locus tag BE845_RS11525 Protein ID WP_011216258.1
Coordinates 2577262..2577570 (-) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE845_RS11505 2571881..2572786 - 906 WP_011216262.1 restriction endonuclease -
BE845_RS11510 2572985..2574358 - 1374 WP_011216261.1 ATP-binding protein -
BE845_RS11515 2574714..2575802 + 1089 WP_011216260.1 DDE-type integrase/transposase/recombinase -
BE845_RS11520 2576327..2577265 - 939 WP_011216259.1 HipA domain-containing protein Toxin
BE845_RS11525 2577262..2577570 - 309 WP_011216258.1 HipA N-terminal domain-containing protein Antitoxin
BE845_RS11530 2577570..2577794 - 225 WP_011216257.1 helix-turn-helix transcriptional regulator -
BE845_RS11535 2578224..2578892 - 669 WP_011216256.1 hypothetical protein -
BE845_RS11540 2578931..2580442 - 1512 WP_129827326.1 Fic family protein -
BE845_RS11550 2580755..2581189 + 435 Protein_2271 hypothetical protein -
BE845_RS11555 2581242..2582429 + 1188 Protein_2272 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - lpg2541 / lpg2539 / lpg2538 / lem27 / lpg2527 / mavL / sidC / sdcA / sdeD/laiF / sdjA / lpg2505 / ceg32/sidI / mavJ / lepB / sdbB / sidD / sidM/drrA / lpg2461 / ankD/legA15 / ankF/legA14/ceg31 / mavI / lpg2443 / lpg2434 / ceg30 / mavH / mavG / lem25 / lpg2420 / legA1 / lem24 / vpdA / ceg29 / lem23 / legL7 / legL6 / sdbC / katB / lpg2382 / lpg2370 2377283..2622169 244886
- flank IS/Tn - - 2571236..2571637 401


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(53-275)

Antitoxin

HipA, N-terminal subdomain 1

(6-98)


Sequences

Toxin        


Download         Length: 313 a.a.        Molecular weight: 36016.80 Da        Isoelectric Point: 9.5712

>T65324 WP_011216259.1 NZ_CP016874:c2577265-2576327 [Legionella pneumophila subsp. pneumophila]
MKRCPITYDLISDQENYSQRGLHLLSPQLKNLSPLDLSADEQRQEAIARVGKMSIQGVQKKLSAKLKIKEGGFEIVDQKG
QYILKPQSDIYPELPENEAITMTLAKAIGLEVPVHGLVYSKDNSLTYFIKRFDRIGHNKKLALEDFAQLSGEDRHTKYKS
SMEKVIAVIQQFCTFPKIEFVKLFKLTLFNFLVGNEDMHLKNFSLITKDRKISISPAYDLINSTIAQKNTKEEIALPLKG
KKNNLTKSDFLKYFAVEKLGLNQNVIDGIVQEFHQIIPEWRKLIGFSFLSQQMQEKYIELLDQRCKRFNFFD

Download         Length: 939 bp

>T65324 NZ_CP016874:c2577265-2576327 [Legionella pneumophila subsp. pneumophila]
ATGAAACGCTGCCCTATTACTTATGACTTAATTAGTGACCAAGAAAACTATTCTCAACGTGGATTGCATTTACTTTCCCC
TCAATTAAAAAACCTTAGCCCATTAGATTTAAGTGCTGATGAGCAGCGACAGGAAGCAATTGCTCGTGTAGGAAAGATGT
CTATTCAAGGCGTCCAAAAGAAACTAAGTGCCAAACTAAAGATTAAGGAGGGAGGTTTTGAAATCGTTGATCAAAAGGGG
CAGTACATTTTAAAACCACAAAGTGATATTTACCCAGAATTGCCGGAAAATGAAGCCATTACTATGACCCTTGCAAAGGC
CATTGGCCTCGAAGTTCCGGTTCATGGTTTGGTTTATTCTAAAGACAACAGTTTGACCTACTTCATTAAACGCTTTGATA
GAATAGGCCATAATAAAAAGTTAGCTTTAGAAGATTTTGCACAGCTATCAGGTGAAGATCGACATACGAAATATAAAAGT
TCTATGGAAAAGGTAATTGCAGTCATACAGCAGTTTTGTACATTCCCAAAAATTGAATTCGTGAAATTATTTAAGCTGAC
GTTGTTTAACTTCCTGGTCGGCAATGAAGACATGCATCTAAAAAATTTTTCCTTAATTACTAAGGATAGAAAAATTTCCA
TATCACCAGCATATGATTTAATTAACTCAACTATCGCTCAAAAAAACACAAAAGAAGAAATCGCTTTGCCTCTGAAAGGG
AAAAAAAATAATTTAACAAAGAGTGATTTTCTTAAATACTTTGCCGTTGAAAAATTAGGATTAAATCAGAACGTCATCGA
TGGGATCGTACAAGAGTTCCATCAAATAATACCTGAATGGCGAAAACTTATTGGTTTCAGTTTCTTATCTCAACAGATGC
AGGAAAAATATATCGAATTGCTCGATCAGCGATGCAAGCGATTCAATTTTTTTGATTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11654.54 Da        Isoelectric Point: 4.6017

>AT65324 WP_011216258.1 NZ_CP016874:c2577570-2577262 [Legionella pneumophila subsp. pneumophila]
MRKAYISVNGIRAGILEELEGKKYQFTYLNDYQGAPVSLTMPLTNKIYDFDVFPPFFEGLLPEGIMLEALLRKYKIDKND
YFGQLIIVGQDVVGAVTIEELR

Download         Length: 309 bp

>AT65324 NZ_CP016874:c2577570-2577262 [Legionella pneumophila subsp. pneumophila]
ATGAGAAAAGCATACATATCAGTGAATGGCATTAGAGCTGGAATATTAGAGGAGTTAGAAGGTAAAAAATATCAATTTAC
TTACCTTAATGATTATCAGGGAGCACCTGTTTCTCTCACCATGCCGTTAACCAATAAGATCTATGATTTTGACGTATTTC
CCCCTTTTTTTGAAGGATTGCTACCTGAAGGTATTATGCTTGAAGCATTATTACGCAAATATAAAATCGATAAAAATGAT
TATTTTGGTCAATTGATAATAGTTGGCCAAGATGTCGTAGGTGCAGTGACGATTGAGGAACTAAGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q5WU78

Antitoxin

Source ID Structure
AlphaFold DB A0A317U6X7

References