65322

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) HipBST/HipA(toxin)
Location 1214920..1216163 Replicon chromosome
Accession NZ_CP016873
Organism Legionella pneumophila subsp. pneumophila strain FFI105

Toxin (Protein)


Gene name HipT Uniprot ID Q5WU78
Locus tag BE844_RS05335 Protein ID WP_011216259.1
Coordinates 1215225..1216163 (+) Length 313 a.a.

Antitoxin (Protein)


Gene name HipS Uniprot ID Q5WU79
Locus tag BE844_RS05330 Protein ID WP_011216258.1
Coordinates 1214920..1215228 (+) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE844_RS05300 1210061..1211248 - 1188 Protein_1028 transposase -
BE844_RS05305 1211301..1211735 - 435 Protein_1029 hypothetical protein -
BE844_RS05315 1212048..1213559 + 1512 WP_129827326.1 Fic family protein -
BE844_RS05320 1213598..1214266 + 669 WP_011216256.1 hypothetical protein -
BE844_RS05325 1214696..1214920 + 225 WP_011216257.1 helix-turn-helix transcriptional regulator -
BE844_RS05330 1214920..1215228 + 309 WP_011216258.1 HipA N-terminal domain-containing protein Antitoxin
BE844_RS05335 1215225..1216163 + 939 WP_011216259.1 HipA domain-containing protein Toxin
BE844_RS05340 1216688..1217776 - 1089 WP_011216260.1 DDE-type integrase/transposase/recombinase -
BE844_RS05345 1218132..1219505 + 1374 WP_011216261.1 ATP-binding protein -
BE844_RS05350 1219704..1220609 + 906 WP_011216262.1 restriction endonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1220853..1221254 401


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(53-275)

Antitoxin

HipA, N-terminal subdomain 1

(6-98)


Sequences

Toxin        


Download         Length: 313 a.a.        Molecular weight: 36016.80 Da        Isoelectric Point: 9.5712

>T65322 WP_011216259.1 NZ_CP016873:1215225-1216163 [Legionella pneumophila subsp. pneumophila]
MKRCPITYDLISDQENYSQRGLHLLSPQLKNLSPLDLSADEQRQEAIARVGKMSIQGVQKKLSAKLKIKEGGFEIVDQKG
QYILKPQSDIYPELPENEAITMTLAKAIGLEVPVHGLVYSKDNSLTYFIKRFDRIGHNKKLALEDFAQLSGEDRHTKYKS
SMEKVIAVIQQFCTFPKIEFVKLFKLTLFNFLVGNEDMHLKNFSLITKDRKISISPAYDLINSTIAQKNTKEEIALPLKG
KKNNLTKSDFLKYFAVEKLGLNQNVIDGIVQEFHQIIPEWRKLIGFSFLSQQMQEKYIELLDQRCKRFNFFD

Download         Length: 939 bp

>T65322 NZ_CP016873:1215225-1216163 [Legionella pneumophila subsp. pneumophila]
ATGAAACGCTGCCCTATTACTTATGACTTAATTAGTGACCAAGAAAACTATTCTCAACGTGGATTGCATTTACTTTCCCC
TCAATTAAAAAACCTTAGCCCATTAGATTTAAGTGCTGATGAGCAGCGACAGGAAGCAATTGCTCGTGTAGGAAAGATGT
CTATTCAAGGCGTCCAAAAGAAACTAAGTGCCAAACTAAAGATTAAGGAGGGAGGTTTTGAAATCGTTGATCAAAAGGGG
CAGTACATTTTAAAACCACAAAGTGATATTTACCCAGAATTGCCGGAAAATGAAGCCATTACTATGACCCTTGCAAAGGC
CATTGGCCTCGAAGTTCCGGTTCATGGTTTGGTTTATTCTAAAGACAACAGTTTGACCTACTTCATTAAACGCTTTGATA
GAATAGGCCATAATAAAAAGTTAGCTTTAGAAGATTTTGCACAGCTATCAGGTGAAGATCGACATACGAAATATAAAAGT
TCTATGGAAAAGGTAATTGCAGTCATACAGCAGTTTTGTACATTCCCAAAAATTGAATTCGTGAAATTATTTAAGCTGAC
GTTGTTTAACTTCCTGGTCGGCAATGAAGACATGCATCTAAAAAATTTTTCCTTAATTACTAAGGATAGAAAAATTTCCA
TATCACCAGCATATGATTTAATTAACTCAACTATCGCTCAAAAAAACACAAAAGAAGAAATCGCTTTGCCTCTGAAAGGG
AAAAAAAATAATTTAACAAAGAGTGATTTTCTTAAATACTTTGCCGTTGAAAAATTAGGATTAAATCAGAACGTCATCGA
TGGGATCGTACAAGAGTTCCATCAAATAATACCTGAATGGCGAAAACTTATTGGTTTCAGTTTCTTATCTCAACAGATGC
AGGAAAAATATATCGAATTGCTCGATCAGCGATGCAAGCGATTCAATTTTTTTGATTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11654.54 Da        Isoelectric Point: 4.6017

>AT65322 WP_011216258.1 NZ_CP016873:1214920-1215228 [Legionella pneumophila subsp. pneumophila]
MRKAYISVNGIRAGILEELEGKKYQFTYLNDYQGAPVSLTMPLTNKIYDFDVFPPFFEGLLPEGIMLEALLRKYKIDKND
YFGQLIIVGQDVVGAVTIEELR

Download         Length: 309 bp

>AT65322 NZ_CP016873:1214920-1215228 [Legionella pneumophila subsp. pneumophila]
ATGAGAAAAGCATACATATCAGTGAATGGCATTAGAGCTGGAATATTAGAGGAGTTAGAAGGTAAAAAATATCAATTTAC
TTACCTTAATGATTATCAGGGAGCACCTGTTTCTCTCACCATGCCGTTAACCAATAAGATCTATGATTTTGACGTATTTC
CCCCTTTTTTTGAAGGATTGCTACCTGAAGGTATTATGCTTGAAGCATTATTACGCAAATATAAAATCGATAAAAATGAT
TATTTTGGTCAATTGATAATAGTTGGCCAAGATGTCGTAGGTGCAGTGACGATTGAGGAACTAAGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q5WU78

Antitoxin

Source ID Structure
AlphaFold DB A0A317U6X7

References