Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) HipBST/HipA(toxin)
Location 2969867..2971110 Replicon chromosome
Accession NZ_CP016872
Organism Legionella pneumophila subsp. pneumophila strain FFI104

Toxin (Protein)


Gene name HipT Uniprot ID Q5WU78
Locus tag BE843_RS13435 Protein ID WP_011216259.1
Coordinates 2969867..2970805 (-) Length 313 a.a.

Antitoxin (Protein)


Gene name HipS Uniprot ID Q5WU79
Locus tag BE843_RS13440 Protein ID WP_011216258.1
Coordinates 2970802..2971110 (-) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE843_RS13420 2965421..2966326 - 906 WP_011216262.1 restriction endonuclease -
BE843_RS13425 2966525..2967898 - 1374 WP_011216261.1 ATP-binding protein -
BE843_RS13430 2968254..2969342 + 1089 WP_011216260.1 DDE-type integrase/transposase/recombinase -
BE843_RS13435 2969867..2970805 - 939 WP_011216259.1 HipA domain-containing protein Toxin
BE843_RS13440 2970802..2971110 - 309 WP_011216258.1 HipA N-terminal domain-containing protein Antitoxin
BE843_RS13445 2971110..2971334 - 225 WP_011216257.1 helix-turn-helix transcriptional regulator -
BE843_RS13450 2971764..2972432 - 669 WP_011216256.1 hypothetical protein -
BE843_RS13455 2972471..2973982 - 1512 WP_129827326.1 Fic family protein -
BE843_RS13465 2974295..2974729 + 435 Protein_2646 hypothetical protein -
BE843_RS13470 2974782..2975969 + 1188 Protein_2647 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2964776..2965177 401


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(53-275)

Antitoxin

HipA, N-terminal subdomain 1

(6-98)


Sequences

Toxin        


Download         Length: 313 a.a.        Molecular weight: 36016.80 Da        Isoelectric Point: 9.5712

>T65320 WP_011216259.1 NZ_CP016872:c2970805-2969867 [Legionella pneumophila subsp. pneumophila]
MKRCPITYDLISDQENYSQRGLHLLSPQLKNLSPLDLSADEQRQEAIARVGKMSIQGVQKKLSAKLKIKEGGFEIVDQKG
QYILKPQSDIYPELPENEAITMTLAKAIGLEVPVHGLVYSKDNSLTYFIKRFDRIGHNKKLALEDFAQLSGEDRHTKYKS
SMEKVIAVIQQFCTFPKIEFVKLFKLTLFNFLVGNEDMHLKNFSLITKDRKISISPAYDLINSTIAQKNTKEEIALPLKG
KKNNLTKSDFLKYFAVEKLGLNQNVIDGIVQEFHQIIPEWRKLIGFSFLSQQMQEKYIELLDQRCKRFNFFD

Download         Length: 939 bp

>T65320 NZ_CP016872:c2970805-2969867 [Legionella pneumophila subsp. pneumophila]
ATGAAACGCTGCCCTATTACTTATGACTTAATTAGTGACCAAGAAAACTATTCTCAACGTGGATTGCATTTACTTTCCCC
TCAATTAAAAAACCTTAGCCCATTAGATTTAAGTGCTGATGAGCAGCGACAGGAAGCAATTGCTCGTGTAGGAAAGATGT
CTATTCAAGGCGTCCAAAAGAAACTAAGTGCCAAACTAAAGATTAAGGAGGGAGGTTTTGAAATCGTTGATCAAAAGGGG
CAGTACATTTTAAAACCACAAAGTGATATTTACCCAGAATTGCCGGAAAATGAAGCCATTACTATGACCCTTGCAAAGGC
CATTGGCCTCGAAGTTCCGGTTCATGGTTTGGTTTATTCTAAAGACAACAGTTTGACCTACTTCATTAAACGCTTTGATA
GAATAGGCCATAATAAAAAGTTAGCTTTAGAAGATTTTGCACAGCTATCAGGTGAAGATCGACATACGAAATATAAAAGT
TCTATGGAAAAGGTAATTGCAGTCATACAGCAGTTTTGTACATTCCCAAAAATTGAATTCGTGAAATTATTTAAGCTGAC
GTTGTTTAACTTCCTGGTCGGCAATGAAGACATGCATCTAAAAAATTTTTCCTTAATTACTAAGGATAGAAAAATTTCCA
TATCACCAGCATATGATTTAATTAACTCAACTATCGCTCAAAAAAACACAAAAGAAGAAATCGCTTTGCCTCTGAAAGGG
AAAAAAAATAATTTAACAAAGAGTGATTTTCTTAAATACTTTGCCGTTGAAAAATTAGGATTAAATCAGAACGTCATCGA
TGGGATCGTACAAGAGTTCCATCAAATAATACCTGAATGGCGAAAACTTATTGGTTTCAGTTTCTTATCTCAACAGATGC
AGGAAAAATATATCGAATTGCTCGATCAGCGATGCAAGCGATTCAATTTTTTTGATTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11654.54 Da        Isoelectric Point: 4.6017

>AT65320 WP_011216258.1 NZ_CP016872:c2971110-2970802 [Legionella pneumophila subsp. pneumophila]
MRKAYISVNGIRAGILEELEGKKYQFTYLNDYQGAPVSLTMPLTNKIYDFDVFPPFFEGLLPEGIMLEALLRKYKIDKND
YFGQLIIVGQDVVGAVTIEELR

Download         Length: 309 bp

>AT65320 NZ_CP016872:c2971110-2970802 [Legionella pneumophila subsp. pneumophila]
ATGAGAAAAGCATACATATCAGTGAATGGCATTAGAGCTGGAATATTAGAGGAGTTAGAAGGTAAAAAATATCAATTTAC
TTACCTTAATGATTATCAGGGAGCACCTGTTTCTCTCACCATGCCGTTAACCAATAAGATCTATGATTTTGACGTATTTC
CCCCTTTTTTTGAAGGATTGCTACCTGAAGGTATTATGCTTGAAGCATTATTACGCAAATATAAAATCGATAAAAATGAT
TATTTTGGTCAATTGATAATAGTTGGCCAAGATGTCGTAGGTGCAGTGACGATTGAGGAACTAAGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q5WU78

Antitoxin

Source ID Structure
AlphaFold DB A0A317U6X7

References