Detailed information of TA system TA065311

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA(toxin)
• Location: 242870..244113
• Replicon: chromosome
• Accession: NZ_CP016868
• Organism: Legionella pneumophila subsp. pneumophila strain FFI102

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: Q5WU78
• Locus tag: BE841_RS01060
• Protein ID: WP_011216259.1
• Coordinates: 242870..243808 (-)
• Length: 313 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: Q5WU79
• Locus tag: BE841_RS01065
• Protein ID: WP_011216258.1
• Coordinates: 243805..244113 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE841_RS01045	238424..239329	-	906	WP_011216262.1	restriction endonuclease	-
BE841_RS01050	239528..240901	-	1374	WP_011216261.1	ATP-binding protein	-
BE841_RS01055	241257..242345	+	1089	WP_011216260.1	DDE-type integrase/transposase/recombinase	-
BE841_RS01060	242870..243808	-	939	WP_011216259.1	HipA domain-containing protein	Toxin
BE841_RS01065	243805..244113	-	309	WP_011216258.1	HipA N-terminal domain-containing protein	Antitoxin
BE841_RS01070	244113..244337	-	225	WP_011216257.1	helix-turn-helix transcriptional regulator	-
BE841_RS01075	244767..245435	-	669	WP_011216256.1	hypothetical protein	-
BE841_RS01080	245474..246985	-	1512	WP_129827326.1	Fic family protein	-
BE841_RS01090	247298..247732	+	435	Protein_215	hypothetical protein	-
BE841_RS01095	247785..248972	+	1188	Protein_216	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	lpg2541 / lpg2539 / lpg2538 / lem27 / lpg2527 / mavL / sidC / sdcA / sdeD/laiF / sdjA / lpg2505 / ceg32/sidI / mavJ / lepB / sdbB / sidD / sidM/drrA / lpg2461 / ankD/legA15 / ankF/legA14/ceg31 / mavI / lpg2443 / lpg2434 / ceg30 / mavH / mavG / lem25 / lpg2420 / legA1 / lem24 / vpdA / ceg29 / lem23 / legL7 / legL6 / sdbC / katB / lpg2382 / lpg2370	43826..288712	244886
	-	flank	IS/Tn	-	-	237779..238180	401

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (53-275)

Antitoxin

• HipA, N-terminal subdomain 1 (6-98)

Sequences

Toxin

Protein

• Length: 313 a.a.
• Molecular weight: 36016.80 Da
• Isoelectric Point: 9.5712

>T65311 WP_011216259.1 NZ_CP016868:c243808-242870 [Legionella pneumophila subsp. pneumophila]
MKRCPITYDLISDQENYSQRGLHLLSPQLKNLSPLDLSADEQRQEAIARVGKMSIQGVQKKLSAKLKIKEGGFEIVDQKG
QYILKPQSDIYPELPENEAITMTLAKAIGLEVPVHGLVYSKDNSLTYFIKRFDRIGHNKKLALEDFAQLSGEDRHTKYKS
SMEKVIAVIQQFCTFPKIEFVKLFKLTLFNFLVGNEDMHLKNFSLITKDRKISISPAYDLINSTIAQKNTKEEIALPLKG
KKNNLTKSDFLKYFAVEKLGLNQNVIDGIVQEFHQIIPEWRKLIGFSFLSQQMQEKYIELLDQRCKRFNFFD

Nucleotide

• Length: 939 bp

>T65311 NZ_CP016868:c243808-242870 [Legionella pneumophila subsp. pneumophila]
ATGAAACGCTGCCCTATTACTTATGACTTAATTAGTGACCAAGAAAACTATTCTCAACGTGGATTGCATTTACTTTCCCC
TCAATTAAAAAACCTTAGCCCATTAGATTTAAGTGCTGATGAGCAGCGACAGGAAGCAATTGCTCGTGTAGGAAAGATGT
CTATTCAAGGCGTCCAAAAGAAACTAAGTGCCAAACTAAAGATTAAGGAGGGAGGTTTTGAAATCGTTGATCAAAAGGGG
CAGTACATTTTAAAACCACAAAGTGATATTTACCCAGAATTGCCGGAAAATGAAGCCATTACTATGACCCTTGCAAAGGC
CATTGGCCTCGAAGTTCCGGTTCATGGTTTGGTTTATTCTAAAGACAACAGTTTGACCTACTTCATTAAACGCTTTGATA
GAATAGGCCATAATAAAAAGTTAGCTTTAGAAGATTTTGCACAGCTATCAGGTGAAGATCGACATACGAAATATAAAAGT
TCTATGGAAAAGGTAATTGCAGTCATACAGCAGTTTTGTACATTCCCAAAAATTGAATTCGTGAAATTATTTAAGCTGAC
GTTGTTTAACTTCCTGGTCGGCAATGAAGACATGCATCTAAAAAATTTTTCCTTAATTACTAAGGATAGAAAAATTTCCA
TATCACCAGCATATGATTTAATTAACTCAACTATCGCTCAAAAAAACACAAAAGAAGAAATCGCTTTGCCTCTGAAAGGG
AAAAAAAATAATTTAACAAAGAGTGATTTTCTTAAATACTTTGCCGTTGAAAAATTAGGATTAAATCAGAACGTCATCGA
TGGGATCGTACAAGAGTTCCATCAAATAATACCTGAATGGCGAAAACTTATTGGTTTCAGTTTCTTATCTCAACAGATGC
AGGAAAAATATATCGAATTGCTCGATCAGCGATGCAAGCGATTCAATTTTTTTGATTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11654.54 Da
• Isoelectric Point: 4.6017

>AT65311 WP_011216258.1 NZ_CP016868:c244113-243805 [Legionella pneumophila subsp. pneumophila]
MRKAYISVNGIRAGILEELEGKKYQFTYLNDYQGAPVSLTMPLTNKIYDFDVFPPFFEGLLPEGIMLEALLRKYKIDKND
YFGQLIIVGQDVVGAVTIEELR

Nucleotide

• Length: 309 bp

>AT65311 NZ_CP016868:c244113-243805 [Legionella pneumophila subsp. pneumophila]
ATGAGAAAAGCATACATATCAGTGAATGGCATTAGAGCTGGAATATTAGAGGAGTTAGAAGGTAAAAAATATCAATTTAC
TTACCTTAATGATTATCAGGGAGCACCTGTTTCTCTCACCATGCCGTTAACCAATAAGATCTATGATTTTGACGTATTTC
CCCCTTTTTTTGAAGGATTGCTACCTGAAGGTATTATGCTTGAAGCATTATTACGCAAATATAAAATCGATAAAAATGAT
TATTTTGGTCAATTGATAATAGTTGGCCAAGATGTCGTAGGTGCAGTGACGATTGAGGAACTAAGATGA

Structures

Toxin

• AlphaFold DB: Q5WU78

Antitoxin

• AlphaFold DB: A0A317U6X7