Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) HipBST/HipA(toxin)
Location 242870..244113 Replicon chromosome
Accession NZ_CP016868
Organism Legionella pneumophila subsp. pneumophila strain FFI102

Toxin (Protein)


Gene name HipT Uniprot ID Q5WU78
Locus tag BE841_RS01060 Protein ID WP_011216259.1
Coordinates 242870..243808 (-) Length 313 a.a.

Antitoxin (Protein)


Gene name HipS Uniprot ID Q5WU79
Locus tag BE841_RS01065 Protein ID WP_011216258.1
Coordinates 243805..244113 (-) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BE841_RS01045 238424..239329 - 906 WP_011216262.1 restriction endonuclease -
BE841_RS01050 239528..240901 - 1374 WP_011216261.1 ATP-binding protein -
BE841_RS01055 241257..242345 + 1089 WP_011216260.1 DDE-type integrase/transposase/recombinase -
BE841_RS01060 242870..243808 - 939 WP_011216259.1 HipA domain-containing protein Toxin
BE841_RS01065 243805..244113 - 309 WP_011216258.1 HipA N-terminal domain-containing protein Antitoxin
BE841_RS01070 244113..244337 - 225 WP_011216257.1 helix-turn-helix transcriptional regulator -
BE841_RS01075 244767..245435 - 669 WP_011216256.1 hypothetical protein -
BE841_RS01080 245474..246985 - 1512 WP_129827326.1 Fic family protein -
BE841_RS01090 247298..247732 + 435 Protein_215 hypothetical protein -
BE841_RS01095 247785..248972 + 1188 Protein_216 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - lpg2541 / lpg2539 / lpg2538 / lem27 / lpg2527 / mavL / sidC / sdcA / sdeD/laiF / sdjA / lpg2505 / ceg32/sidI / mavJ / lepB / sdbB / sidD / sidM/drrA / lpg2461 / ankD/legA15 / ankF/legA14/ceg31 / mavI / lpg2443 / lpg2434 / ceg30 / mavH / mavG / lem25 / lpg2420 / legA1 / lem24 / vpdA / ceg29 / lem23 / legL7 / legL6 / sdbC / katB / lpg2382 / lpg2370 43826..288712 244886
- flank IS/Tn - - 237779..238180 401


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(53-275)

Antitoxin

HipA, N-terminal subdomain 1

(6-98)


Sequences

Toxin        


Download         Length: 313 a.a.        Molecular weight: 36016.80 Da        Isoelectric Point: 9.5712

>T65311 WP_011216259.1 NZ_CP016868:c243808-242870 [Legionella pneumophila subsp. pneumophila]
MKRCPITYDLISDQENYSQRGLHLLSPQLKNLSPLDLSADEQRQEAIARVGKMSIQGVQKKLSAKLKIKEGGFEIVDQKG
QYILKPQSDIYPELPENEAITMTLAKAIGLEVPVHGLVYSKDNSLTYFIKRFDRIGHNKKLALEDFAQLSGEDRHTKYKS
SMEKVIAVIQQFCTFPKIEFVKLFKLTLFNFLVGNEDMHLKNFSLITKDRKISISPAYDLINSTIAQKNTKEEIALPLKG
KKNNLTKSDFLKYFAVEKLGLNQNVIDGIVQEFHQIIPEWRKLIGFSFLSQQMQEKYIELLDQRCKRFNFFD

Download         Length: 939 bp

>T65311 NZ_CP016868:c243808-242870 [Legionella pneumophila subsp. pneumophila]
ATGAAACGCTGCCCTATTACTTATGACTTAATTAGTGACCAAGAAAACTATTCTCAACGTGGATTGCATTTACTTTCCCC
TCAATTAAAAAACCTTAGCCCATTAGATTTAAGTGCTGATGAGCAGCGACAGGAAGCAATTGCTCGTGTAGGAAAGATGT
CTATTCAAGGCGTCCAAAAGAAACTAAGTGCCAAACTAAAGATTAAGGAGGGAGGTTTTGAAATCGTTGATCAAAAGGGG
CAGTACATTTTAAAACCACAAAGTGATATTTACCCAGAATTGCCGGAAAATGAAGCCATTACTATGACCCTTGCAAAGGC
CATTGGCCTCGAAGTTCCGGTTCATGGTTTGGTTTATTCTAAAGACAACAGTTTGACCTACTTCATTAAACGCTTTGATA
GAATAGGCCATAATAAAAAGTTAGCTTTAGAAGATTTTGCACAGCTATCAGGTGAAGATCGACATACGAAATATAAAAGT
TCTATGGAAAAGGTAATTGCAGTCATACAGCAGTTTTGTACATTCCCAAAAATTGAATTCGTGAAATTATTTAAGCTGAC
GTTGTTTAACTTCCTGGTCGGCAATGAAGACATGCATCTAAAAAATTTTTCCTTAATTACTAAGGATAGAAAAATTTCCA
TATCACCAGCATATGATTTAATTAACTCAACTATCGCTCAAAAAAACACAAAAGAAGAAATCGCTTTGCCTCTGAAAGGG
AAAAAAAATAATTTAACAAAGAGTGATTTTCTTAAATACTTTGCCGTTGAAAAATTAGGATTAAATCAGAACGTCATCGA
TGGGATCGTACAAGAGTTCCATCAAATAATACCTGAATGGCGAAAACTTATTGGTTTCAGTTTCTTATCTCAACAGATGC
AGGAAAAATATATCGAATTGCTCGATCAGCGATGCAAGCGATTCAATTTTTTTGATTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11654.54 Da        Isoelectric Point: 4.6017

>AT65311 WP_011216258.1 NZ_CP016868:c244113-243805 [Legionella pneumophila subsp. pneumophila]
MRKAYISVNGIRAGILEELEGKKYQFTYLNDYQGAPVSLTMPLTNKIYDFDVFPPFFEGLLPEGIMLEALLRKYKIDKND
YFGQLIIVGQDVVGAVTIEELR

Download         Length: 309 bp

>AT65311 NZ_CP016868:c244113-243805 [Legionella pneumophila subsp. pneumophila]
ATGAGAAAAGCATACATATCAGTGAATGGCATTAGAGCTGGAATATTAGAGGAGTTAGAAGGTAAAAAATATCAATTTAC
TTACCTTAATGATTATCAGGGAGCACCTGTTTCTCTCACCATGCCGTTAACCAATAAGATCTATGATTTTGACGTATTTC
CCCCTTTTTTTGAAGGATTGCTACCTGAAGGTATTATGCTTGAAGCATTATTACGCAAATATAAAATCGATAAAAATGAT
TATTTTGGTCAATTGATAATAGTTGGCCAAGATGTCGTAGGTGCAGTGACGATTGAGGAACTAAGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q5WU78

Antitoxin

Source ID Structure
AlphaFold DB A0A317U6X7

References