Detailed information of TA system TA065222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 64781..65195
• Replicon: chromosome
• Accession: NZ_CP016855
• Organism: Staphylococcus aureus strain 5118.N

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: A7U51_RS00705
• Protein ID: WP_031895065.1
• Coordinates: 64781..65032 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A7U51_RS15460
• Protein ID: WP_000028424.1
• Coordinates: 65022..65195 (+)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7U51_RS00660	60391..61173	+	783	WP_031880075.1	AP2 domain-containing protein	-
A7U51_RS00665	61145..61948	+	804	WP_000504989.1	phage replisome organizer N-terminal domain-containing protein	-
A7U51_RS00670	61958..62737	+	780	WP_000803070.1	ATP-binding protein	-
A7U51_RS15455	62731..62892	+	162	WP_000237154.1	hypothetical protein	-
A7U51_RS00675	62905..63126	+	222	WP_001123688.1	DUF3269 family protein	-
A7U51_RS00680	63136..63540	+	405	WP_031880025.1	DUF1064 domain-containing protein	-
A7U51_RS00685	63545..63730	+	186	WP_001187243.1	DUF3113 family protein	-
A7U51_RS00690	63731..64036	+	306	WP_000101252.1	hypothetical protein	-
A7U51_RS00695	64164..64520	+	357	WP_000029376.1	hypothetical protein	-
A7U51_RS00700	64524..64766	+	243	WP_001562543.1	phage protein	-
A7U51_RS00705	64781..65032	+	252	WP_031895065.1	DUF1024 family protein	Toxin
A7U51_RS15460	65022..65195	+	174	WP_000028424.1	hypothetical protein	Antitoxin
A7U51_RS00710	65196..65477	+	282	WP_000454994.1	hypothetical protein	-
A7U51_RS15465	65478..65636	+	159	WP_000889681.1	hypothetical protein	-
A7U51_RS00715	65654..66190	+	537	WP_031892660.1	dUTPase	-
A7U51_RS00720	66227..66415	+	189	WP_000195782.1	DUF1381 domain-containing protein	-
A7U51_RS00725	66390..66590	+	201	WP_001125015.1	hypothetical protein	-
A7U51_RS00730	66593..66766	+	174	WP_000237865.1	transcriptional activator RinB	-
A7U51_RS00735	66767..66913	+	147	WP_000989998.1	hypothetical protein	-
A7U51_RS00740	66937..67359	+	423	WP_000162699.1	RinA family phage transcriptional activator	-
A7U51_RS00745	67546..67986	+	441	WP_001003272.1	terminase small subunit	-
A7U51_RS00750	67973..69250	+	1278	WP_031895010.1	PBSX family phage terminase large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	51732..96033	44301

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1024 (1-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9469.40 Da
• Isoelectric Point: 3.7415

>T65222 WP_031895065.1 NZ_CP016855:64781-65032 [Staphylococcus aureus]
MNNREQIEQSIISASAYNGNDTEGLLKEIEDVYKKAQAFDEILEGLPNAMQDALKEDIYLDEAVGIMTSQVVYKYEEAQE
NEY

Nucleotide

• Length: 252 bp

>T65222 NZ_CP016855:64781-65032 [Staphylococcus aureus]
ATGAATAACCGCGAACAAATTGAACAATCAATTATCAGTGCTAGTGCGTATAACGGCAATGACACAGAGGGATTACTAAA
AGAGATTGAAGATGTGTATAAGAAAGCGCAAGCGTTTGATGAAATACTTGAGGGTTTACCTAATGCTATGCAAGATGCAC
TCAAAGAAGATATTTATCTTGATGAAGCAGTAGGGATTATGACGAGTCAAGTGGTCTATAAATATGAGGAGGCGCAGGAA
AATGAGTATTAG

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6468.29 Da
• Isoelectric Point: 4.2484

>AT65222 WP_000028424.1 NZ_CP016855:65022-65195 [Staphylococcus aureus]
MSISVGDKVYNHETNESLEIVQLVGDIRDTHYKLSDDSVISIIDFITKPIYLIKGDE

Nucleotide

• Length: 174 bp

>AT65222 NZ_CP016855:65022-65195 [Staphylococcus aureus]
ATGAGTATTAGTGTAGGAGATAAAGTATATAACCATGAAACAAACGAAAGTCTAGAGATTGTGCAATTGGTCGGAGATAT
TAGAGATACACATTATAAACTGTCTGATGATTCAGTTATTAGCATTATAGATTTTATTACTAAACCAATTTATCTAATTA
AGGGGGACGAGTGA