Detailed information of TA system TA065201

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): bsrE-as-bsrE/-
• Location: 2069230..2069545
• Replicon: chromosome
• Accession: NZ_CP016852
• Organism: Bacillus subtilis subsp. subtilis strain 168G

Toxin (Protein)

• Gene name: bsrE
• Uniprot ID: -
• Locus tag: BFI33_RS22795
• Protein ID: WP_120322743.1
• Coordinates: 2069230..2069391 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	as-bsrE
Locus tag	-
Coordinates	2069372..2069545 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFI33_RS10435	2064458..2064793	+	336	WP_009967409.1	hypothetical protein	-
BFI33_RS10440	2064840..2068445	-	3606	WP_004399367.1	membrane protein	-
BFI33_RS10445	2068678..2069010	-	333	WP_026009814.1	hypothetical protein	-
BFI33_RS22795	2069230..2069391	+	162	WP_120322743.1	putative holin-like toxin	Toxin
-	2069372..2069545	-	174	-	-	Antitoxin
BFI33_RS10450	2069660..2070502	-	843	WP_003231327.1	hypothetical protein	-
BFI33_RS10455	2070702..2071160	-	459	WP_003231326.1	type II toxin-antitoxin system antitoxin YobK	-
BFI33_RS10460	2071170..2072972	-	1803	WP_004399481.1	type II toxin-antitoxin system toxin ribonuclease YobL	-
BFI33_RS10465	2073074..2073631	-	558	WP_004399321.1	SMI1/KNR4 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2055284..2080510	25226

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (24-47)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5780.74 Da
• Isoelectric Point: 4.6641

>T65201 WP_120322743.1 NZ_CP016852:2069230-2069391 [Bacillus subtilis subsp. subtilis]
ISCTVSFAHSRESCSSPNERGDAMSTFQALMLMLAIGSFIIALLTYIEKIDLP

Nucleotide

• Length: 162 bp

>T65201 NZ_CP016852:2069230-2069391 [Bacillus subtilis subsp. subtilis]
ATTTCCTGTACAGTAAGTTTTGCTCACTCGAGGGAGTCTTGCTCATCCCCTAATGAAAGGGGTGATGCTATGTCAACATT
CCAGGCATTAATGCTAATGCTTGCAATCGGGTCGTTTATAATCGCCCTATTGACGTATATAGAAAAAATAGACCTCCCTT
GA

Antitoxin

Nucleotide

• Length: 174 bp

>AT65201 NZ_CP016852:c2069545-2069372 [Bacillus subtilis subsp. subtilis]
TTACAATACATTACAGTGCATTTCTTTAATGAGAAATGCATAAAATAAAAAAGACCGAGGTGCTACCAACACCCCGGCTC
TGTACAAAAAGTTGCCCATCAAAGGGCTTGCTCGATGTGGTTCTAGGTAGGCCTATCCCTTCAGGTTCTGAGGCTCAAGG
GAGGTCTATTTTTT

Structures

Antitoxin