Detailed information of TA system TA065195

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3671529..3672192
• Replicon: chromosome
• Accession: NZ_CP016849
• Organism: Pseudomonas sp. TMW 2.1634

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: BFC21_RS17325
• Protein ID: WP_088378491.1
• Coordinates: 3671827..3672192 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A494R510
• Locus tag: BFC21_RS17320
• Protein ID: WP_016783069.1
• Coordinates: 3671529..3671834 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFC21_RS17295	3666894..3667301	-	408	WP_095004377.1	type II toxin-antitoxin system HicB family antitoxin	-
BFC21_RS17300	3667342..3667521	-	180	WP_095004378.1	type II toxin-antitoxin system HicA family toxin	-
BFC21_RS17305	3667755..3669095	+	1341	WP_088378488.1	gluconate:H+ symporter	-
BFC21_RS17310	3669139..3670485	+	1347	WP_095004380.1	D-serine ammonia-lyase	-
BFC21_RS17315	3670569..3671522	+	954	WP_120266394.1	DNA-binding transcriptional regulator DsdC	-
BFC21_RS17320	3671529..3671834	-	306	WP_016783069.1	XRE family transcriptional regulator	Antitoxin
BFC21_RS17325	3671827..3672192	-	366	WP_088378491.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BFC21_RS17330	3672409..3673692	-	1284	WP_086799352.1	OprD family porin	-
BFC21_RS17335	3673942..3674637	-	696	WP_120266395.1	DsbA family protein	-
BFC21_RS17340	3674896..3676974	+	2079	WP_088378493.1	TonB-dependent siderophore receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (16-86)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 14081.89 Da
• Isoelectric Point: 6.6378

>T65195 WP_088378491.1 NZ_CP016849:c3672192-3671827 [Pseudomonas sp. TMW 2.1634]
MTWNVEYTDEFGYWWERLSEKEQVSVAASVKLLELLGPALRFPHCSDIKGSRHGNLRELRVQHAGRPYRVLYAFDPRRCA
LLLIGGDKTGHDRWYEENVPVAQRLYDVHLQTLIEEGQNNG

Nucleotide

• Length: 366 bp

>T65195 NZ_CP016849:c3672192-3671827 [Pseudomonas sp. TMW 2.1634]
ATGACATGGAACGTTGAGTACACCGACGAATTTGGCTACTGGTGGGAAAGGCTCAGTGAAAAGGAACAGGTCAGCGTGGC
GGCCAGTGTCAAATTGCTAGAGCTGCTCGGGCCGGCCCTGCGCTTCCCCCATTGCAGCGATATCAAGGGTTCGCGGCATG
GCAACCTTCGAGAGCTGCGTGTACAGCATGCGGGCCGCCCTTATAGGGTGCTTTATGCCTTTGACCCGCGTCGCTGCGCC
TTGCTGTTGATTGGCGGTGACAAGACCGGCCATGACCGGTGGTATGAAGAGAACGTCCCTGTTGCACAAAGGCTTTACGA
CGTTCATTTACAAACACTGATTGAAGAGGGCCAGAATAATGGCTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11395.13 Da
• Isoelectric Point: 7.2023

>AT65195 WP_016783069.1 NZ_CP016849:c3671834-3671529 [Pseudomonas sp. TMW 2.1634]
MAKKFSGLEARMSPEARAQAQALYVQHLQEMPLHELRKAQALSQETLAKALNINQAAVSKMERRTDMYISTLRNYIQAMG
GELEIIATFPDGQIKIENFAE

Nucleotide

• Length: 306 bp

>AT65195 NZ_CP016849:c3671834-3671529 [Pseudomonas sp. TMW 2.1634]
ATGGCTAAAAAATTTTCCGGGCTTGAAGCGCGCATGAGCCCAGAGGCGCGGGCGCAAGCCCAGGCTTTGTACGTCCAGCA
TTTACAAGAAATGCCGTTGCATGAGCTGCGCAAAGCCCAGGCGCTCAGTCAGGAAACGCTGGCCAAAGCACTGAATATCA
ACCAGGCAGCCGTTTCTAAAATGGAGCGGCGTACGGACATGTATATCAGTACGCTGCGCAATTACATCCAGGCCATGGGG
GGCGAGCTGGAAATTATCGCGACCTTTCCGGATGGTCAGATCAAAATCGAAAACTTTGCTGAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A494R510