Detailed information of TA system TA065188

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4196894..4197484
• Replicon: chromosome
• Accession: NZ_CP016833
• Organism: Xanthomonas fragariae isolate Fap29

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A1Y6HPD3
• Locus tag: BER93_RS19235
• Protein ID: WP_002803897.1
• Coordinates: 4197203..4197484 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: BER93_RS19230
• Protein ID: WP_002803896.1
• Coordinates: 4196894..4197184 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BER93_RS19215	4192399..4193367	-	969	WP_065975413.1	IS5 family transposase	-
BER93_RS21600	4193612..4194625	-	1014	WP_082244192.1	hypothetical protein	-
BER93_RS19220	4194806..4195408	-	603	WP_083215096.1	transposase	-
BER93_RS22650	4195514..4195816	-	303	WP_145954077.1	hypothetical protein	-
BER93_RS19225	4196018..4196350	+	333	WP_002803887.1	hypothetical protein	-
BER93_RS19230	4196894..4197184	-	291	WP_002803896.1	HigA family addiction module antidote protein	Antitoxin
BER93_RS19235	4197203..4197484	-	282	WP_002803897.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BER93_RS19240	4197948..4200023	-	2076	WP_065975458.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4145481..4205556	60075

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (7-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-94)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10897.61 Da
• Isoelectric Point: 9.7550

>T65188 WP_002803897.1 NZ_CP016833:c4197484-4197203 [Xanthomonas fragariae]
MIKSIVDKEAEKIWVGERSRRLPADIQSVARRKLRMLNAAAHLDDLRIPPANRLEALKGERRGQYSIRINDQLRICFRWM
EGDVAEVEIVDYH

Nucleotide

• Length: 282 bp

>T65188 NZ_CP016833:c4197484-4197203 [Xanthomonas fragariae]
ATGATCAAGAGCATTGTCGACAAGGAAGCCGAAAAGATCTGGGTGGGTGAGCGCTCCCGCCGGTTGCCGGCCGACATCCA
GTCGGTCGCGCGCCGCAAGTTGCGCATGCTCAACGCTGCCGCGCACCTCGACGATCTGCGCATTCCGCCTGCCAACCGGC
TGGAAGCGTTGAAGGGCGAGCGGCGCGGGCAGTACAGCATCCGGATCAACGACCAGTTGCGCATCTGCTTCCGATGGATG
GAGGGCGATGTCGCCGAGGTCGAAATTGTCGATTACCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10528.25 Da
• Isoelectric Point: 7.1667

>AT65188 WP_002803896.1 NZ_CP016833:c4197184-4196894 [Xanthomonas fragariae]
MTVLPNIHPGEILLEEFLEPMGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALCTTERFWLGLQADYELEQA
HRALGDLPSRIKRLAA

Nucleotide

• Length: 291 bp

>AT65188 NZ_CP016833:c4197184-4196894 [Xanthomonas fragariae]
ATGACAGTCCTACCCAACATCCACCCTGGTGAAATCCTGCTTGAGGAGTTCCTCGAGCCGATGGGCATCAGCCAGAACGC
ATTGGCGCGGGCCACCGGTGTGCCGCCGCGCCGTATCAACGAAATCGTGTTGGGCAAGCGCGGCATCACCGCCGACACCG
CCGTGCGCCTGGCTGCAGCGCTGTGCACGACCGAGCGCTTCTGGCTAGGCCTGCAAGCCGATTACGAGCTGGAACAAGCG
CACCGCGCGCTGGGCGACCTGCCATCGCGGATCAAGCGGCTGGCGGCTTGA

Structures

Toxin

• AlphaFold DB: A0A1Y6HPD3