Detailed information of TA system TA065181

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5042653..5043255
• Replicon: chromosome
• Accession: NZ_CP016828
• Organism: Escherichia coli strain FORC_043

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: FORC43_RS26550
• Protein ID: WP_000897305.1
• Coordinates: 5042944..5043255 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FORC43_RS26545
• Protein ID: WP_000356397.1
• Coordinates: 5042653..5042943 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC43_RS26515	5038597..5039499	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
FORC43_RS26520	5039496..5040131	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
FORC43_RS26525	5040128..5041057	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
FORC43_RS26530	5041387..5041629	-	243	WP_001087409.1	hypothetical protein	-
FORC43_RS26535	5041849..5042067	-	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
FORC43_RS26545	5042653..5042943	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
FORC43_RS26550	5042944..5043255	-	312	WP_000897305.1	hypothetical protein	Toxin
FORC43_RS26555	5043484..5044392	+	909	WP_001162704.1	alpha/beta hydrolase	-
FORC43_RS26560	5044456..5045397	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
FORC43_RS26565	5045442..5045879	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FORC43_RS26570	5045876..5046748	-	873	WP_000920762.1	virulence factor BrkB family protein	-
FORC43_RS26575	5046742..5047341	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T65181 WP_000897305.1 NZ_CP016828:c5043255-5042944 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T65181 NZ_CP016828:c5043255-5042944 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT65181 WP_000356397.1 NZ_CP016828:c5042943-5042653 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT65181 NZ_CP016828:c5042943-5042653 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTCAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7