Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1895757..1896259 Replicon chromosome
Accession NZ_CP016828
Organism Escherichia coli strain FORC_043

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FORC43_RS09620 Protein ID WP_000767829.1
Coordinates 1896005..1896259 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FORC43_RS09615 Protein ID WP_001259255.1
Coordinates 1895757..1896008 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC43_RS09585 1890934..1892001 - 1068 WP_000080087.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
FORC43_RS09590 1892001..1893071 - 1071 WP_000108967.1 histidinol-phosphate transaminase -
FORC43_RS09595 1893068..1894372 - 1305 WP_001386882.1 histidinol dehydrogenase -
FORC43_RS09600 1894378..1895277 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
FORC43_RS09610 1895423..1895473 - 51 WP_001364200.1 his operon leader peptide -
FORC43_RS09615 1895757..1896008 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FORC43_RS09620 1896005..1896259 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FORC43_RS09625 1896342..1897166 + 825 WP_000754758.1 SDR family oxidoreductase -
FORC43_RS09630 1897212..1898141 + 930 WP_000803366.1 LysR family transcriptional regulator -
FORC43_RS09635 1898356..1898418 + 63 WP_010723108.1 membrane protein YoeI -
FORC43_RS09640 1898408..1899766 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FORC43_RS09645 1899945..1901003 + 1059 WP_000492324.1 transport protein YeeE -
FORC43_RS09650 1901017..1901244 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T65163 WP_000767829.1 NZ_CP016828:1896005-1896259 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T65163 NZ_CP016828:1896005-1896259 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT65163 WP_001259255.1 NZ_CP016828:1895757-1896008 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT65163 NZ_CP016828:1895757-1896008 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References