Detailed information of TA system TA065150

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 37931..38658
• Replicon: plasmid unamed
• Accession: NZ_CP016815
• Organism: Klebsiella pneumoniae strain ED23

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: BCV49_RS26690
• Protein ID: WP_011251285.1
• Coordinates: 38347..38658 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BCV49_RS26685
• Protein ID: WP_011251286.1
• Coordinates: 37931..38350 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCV49_RS26665	33671..34620	-	950	Protein_36	DUF1173 family protein	-
BCV49_RS26670	34798..35292	+	495	WP_004212794.1	thermonuclease family protein	-
BCV49_RS26675	35629..36027	+	399	WP_004212796.1	H-NS histone family protein	-
BCV49_RS28860	36345..36758	-	414	Protein_39	aldo/keto reductase	-
BCV49_RS26680	36816..37784	-	969	WP_077270001.1	IS5 family transposase	-
BCV49_RS26685	37931..38350	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
BCV49_RS26690	38347..38658	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
BCV49_RS26695	38863..39321	-	459	Protein_43	DDE-type integrase/transposase/recombinase	-
BCV49_RS26700	39435..40132	+	698	Protein_44	IS1 family transposase	-
BCV49_RS26705	40149..40583	+	435	Protein_45	thioredoxin fold domain-containing protein	-
BCV49_RS26710	40600..40911	+	312	WP_011251282.1	hypothetical protein	-
BCV49_RS26715	40925..41266	+	342	WP_011251281.1	hypothetical protein	-
BCV49_RS26720	41326..42282	+	957	WP_011251280.1	DsbA family protein	-
BCV49_RS26725	42399..42974	+	576	WP_011251279.1	hypothetical protein	-
BCV49_RS26730	43082..43597	+	516	WP_041937820.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / rmpA / iroN / iroD / iroC / iroB / rmpA2 / iutA / iucD / iucC / iucB / iucA	1..212770	212770
	-	inside	IScluster/Tn	-	-	31782..40132	8350

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T65150 WP_011251285.1 NZ_CP016815:c38658-38347 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T65150 NZ_CP016815:c38658-38347 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT65150 WP_011251286.1 NZ_CP016815:c38350-37931 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT65150 NZ_CP016815:c38350-37931 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1