Detailed information of TA system TA065108

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 2781038..2781635
• Replicon: chromosome
• Accession: NZ_CP016811
• Organism: Klebsiella pneumoniae strain DHQP1002001

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: A8C02_RS15480
• Protein ID: WP_004893639.1
• Coordinates: 2781318..2781635 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: A8C02_RS15475
• Protein ID: WP_004142561.1
• Coordinates: 2781038..2781325 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8C02_RS15445	2777118..2777366	+	249	WP_032410665.1	DUF1158 domain-containing protein	-
A8C02_RS15450	2777384..2777725	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
A8C02_RS15455	2777756..2778871	-	1116	Protein_2674	MBL fold metallo-hydrolase	-
A8C02_RS15460	2779051..2779632	+	582	WP_032410661.1	TetR/AcrR family transcriptional regulator	-
A8C02_RS15465	2779632..2780000	+	369	WP_032421096.1	MmcQ/YjbR family DNA-binding protein	-
A8C02_RS15470	2780120..2780773	+	654	WP_068815271.1	oxygen-insensitive NAD(P)H nitroreductase	-
A8C02_RS15475	2781038..2781325	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
A8C02_RS15480	2781318..2781635	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A8C02_RS15485	2781820..2782863	-	1044	WP_032410657.1	DUF2157 domain-containing protein	-
A8C02_RS15490	2783529..2784395	-	867	WP_068815272.1	helix-turn-helix transcriptional regulator	-
A8C02_RS15495	2784504..2785931	+	1428	WP_009308097.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T65108 WP_004893639.1 NZ_CP016811:c2781635-2781318 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T65108 NZ_CP016811:c2781635-2781318 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGTCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT65108 WP_004142561.1 NZ_CP016811:c2781325-2781038 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT65108 NZ_CP016811:c2781325-2781038 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1