65080

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 2972418..2973066 Replicon chromosome
Accession NZ_CP016794
Organism Mycobacterium tuberculosis strain SCAID 320.0

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag BEE65_RS14030 Protein ID WP_003899414.1
Coordinates 2972418..2972741 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag BEE65_RS14035 Protein ID WP_003899415.1
Coordinates 2972821..2973066 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BEE65_RS14000 2967685..2968149 + 465 WP_003900539.1 ParB N-terminal domain-containing protein -
BEE65_RS22190 2968231..2969250 + 1020 Protein_2736 IS3 family transposase -
BEE65_RS14015 2969627..2971066 - 1440 WP_003901443.1 phage major capsid protein -
BEE65_RS14020 2971074..2971607 - 534 WP_003899412.1 HK97 family phage prohead protease -
BEE65_RS21590 2971760..2972251 - 492 WP_003900541.1 phage terminase small subunit P27 family -
BEE65_RS14030 2972418..2972741 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
BEE65_RS14035 2972821..2973066 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
BEE65_RS14040 2973063..2974490 - 1428 WP_003899416.1 DUF3631 domain-containing protein -
BEE65_RS14045 2974492..2974884 - 393 WP_003899417.1 DUF2742 domain-containing protein -
BEE65_RS14050 2974881..2975141 - 261 WP_003899418.1 helix-turn-helix domain-containing protein -
BEE65_RS14055 2975158..2975520 - 363 WP_003900543.1 hypothetical protein -
BEE65_RS14060 2975523..2976650 - 1128 WP_003899420.1 site-specific integrase -
BEE65_RS14065 2976795..2977022 - 228 WP_003899421.1 hypothetical protein -
BEE65_RS21595 2977019..2977408 - 390 WP_003899422.1 hypothetical protein -
BEE65_RS14070 2977314..2977586 + 273 WP_003900544.1 hypothetical protein -
BEE65_RS14075 2977685..2977918 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2966673..2976650 9977


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-82)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-72)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T65080 WP_003899414.1 NZ_CP016794:c2972741-2972418 [Mycobacterium tuberculosis]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T65080 NZ_CP016794:c2972741-2972418 [Mycobacterium tuberculosis]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT65080 WP_003899415.1 NZ_CP016794:c2973066-2972821 [Mycobacterium tuberculosis]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT65080 NZ_CP016794:c2973066-2972821 [Mycobacterium tuberculosis]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References