Detailed information of TA system TA065017

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 4754750..4755162
• Replicon: chromosome
• Accession: NZ_CP016755
• Organism: Escherichia coli strain FORC_044

Toxin (Protein)

• Gene name: symE
• Uniprot ID: Q8FA88
• Locus tag: FORC44_RS25760
• Protein ID: WP_000132630.1
• Coordinates: 4754821..4755162 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	4754750..4754826 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC44_RS25750	4751377..4752846	+	1470	WP_001302468.1	type I restriction-modification system subunit M	-
FORC44_RS25755	4752846..4754600	+	1755	WP_000110082.1	restriction endonuclease subunit S	-
-	4754750..4754826	-	77	NuclAT_15	-	Antitoxin
-	4754750..4754826	-	77	NuclAT_15	-	Antitoxin
-	4754750..4754826	-	77	NuclAT_15	-	Antitoxin
-	4754750..4754826	-	77	NuclAT_15	-	Antitoxin
-	4754750..4754826	-	77	NuclAT_16	-	Antitoxin
-	4754750..4754826	-	77	NuclAT_16	-	Antitoxin
-	4754750..4754826	-	77	NuclAT_16	-	Antitoxin
-	4754750..4754826	-	77	NuclAT_16	-	Antitoxin
FORC44_RS25760	4754821..4755162	+	342	WP_000132630.1	endoribonuclease SymE	Toxin
FORC44_RS25765	4755209..4756372	-	1164	WP_001304006.1	DUF1524 domain-containing protein	-
FORC44_RS25770	4756420..4757301	-	882	WP_001304007.1	DUF262 domain-containing protein	-
FORC44_RS25775	4757398..4757562	-	165	WP_000394274.1	DUF1127 domain-containing protein	-
FORC44_RS25785	4757739..4759151	+	1413	WP_000199295.1	PLP-dependent aminotransferase family protein	-
FORC44_RS31675	4759394..4759594	-	201	WP_001310455.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002655 Escherichia coli O157:H7 str. EDL933 5456776..5475497 0.003 View NC_002695 Escherichia coli O157:H7 str. Sakai 5426911..5445631 0.003	inside	Genomic island	-	espX6	4746949..4768182	21233

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12294.13 Da
• Isoelectric Point: 8.4982

>T65017 WP_000132630.1 NZ_CP016755:4754821-4755162 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T65017 NZ_CP016755:4754821-4755162 [Escherichia coli]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT65017 NZ_CP016755:c4754826-4754750 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0E0Y5T0

Antitoxin