Detailed information of TA system TA065014

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4403420..4404114
• Replicon: chromosome
• Accession: NZ_CP016755
• Organism: Escherichia coli strain FORC_044

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: FORC44_RS24065
• Protein ID: WP_001263495.1
• Coordinates: 4403420..4403818 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: FORC44_RS24070
• Protein ID: WP_000554757.1
• Coordinates: 4403821..4404114 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4399007..4399087	-	81	NuclAT_12	-	-
-	4399007..4399087	-	81	NuclAT_12	-	-
-	4399007..4399087	-	81	NuclAT_12	-	-
-	4399007..4399087	-	81	NuclAT_12	-	-
FORC44_RS24040	4399683..4400141	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
FORC44_RS24045	4400402..4401859	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
FORC44_RS24050	4401916..4402437	-	522	Protein_4369	peptide chain release factor H	-
FORC44_RS24055	4402436..4402639	-	204	Protein_4370	RNA ligase RtcB family protein	-
FORC44_RS24060	4402958..4403410	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
FORC44_RS24065	4403420..4403818	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FORC44_RS24070	4403821..4404114	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FORC44_RS24075	4404166..4405221	-	1056	WP_001226188.1	DNA polymerase IV	-
FORC44_RS24080	4405292..4406077	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
FORC44_RS24085	4406049..4407761	+	1713	Protein_4376	flagellar biosynthesis protein FlhA	-
FORC44_RS24090	4407906..4408403	-	498	Protein_4377	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T65014 WP_001263495.1 NZ_CP016755:c4403818-4403420 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T65014 NZ_CP016755:c4403818-4403420 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT65014 WP_000554757.1 NZ_CP016755:c4404114-4403821 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT65014 NZ_CP016755:c4404114-4403821 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6