Detailed information of TA system TA065005

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2554094..2554465
• Replicon: chromosome
• Accession: NZ_CP016755
• Organism: Escherichia coli strain FORC_044

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: FORC44_RS13665
• Protein ID: WP_001443846.1
• Coordinates: 2554316..2554465 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2554094..2554272 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC44_RS13635	2549846..2550016	+	171	WP_001625136.1	hypothetical protein	-
FORC44_RS13640	2550049..2551422	+	1374	WP_000123746.1	ATP-dependent RNA helicase DbpA	-
FORC44_RS13645	2551551..2552486	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
FORC44_RS13650	2552538..2553773	-	1236	WP_000040839.1	site-specific integrase	-
FORC44_RS13655	2553775..2553990	-	216	WP_000079604.1	excisionase XisR	-
FORC44_RS13660	2554090..2554278	-	189	WP_001302840.1	DUF1187 family protein	-
-	2554094..2554272	+	179	NuclAT_0	-	Antitoxin
-	2554094..2554272	+	179	NuclAT_0	-	Antitoxin
-	2554094..2554272	+	179	NuclAT_0	-	Antitoxin
-	2554094..2554272	+	179	NuclAT_0	-	Antitoxin
FORC44_RS13665	2554316..2554465	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
FORC44_RS13670	2554521..2555330	-	810	WP_000166313.1	recombination protein RecT	-
FORC44_RS13675	2555323..2557923	-	2601	WP_000105140.1	exodeoxyribonuclease VIII	-
FORC44_RS13680	2558025..2558300	-	276	WP_001344816.1	hypothetical protein	-
FORC44_RS13685	2558375..2558545	-	171	WP_001352098.1	conserved protein, Rac prophage	-
FORC44_RS13690	2558545..2558766	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI / nleH2 / nleF	2539472..2660345	120873

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T65005 WP_001443846.1 NZ_CP016755:c2554465-2554316 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T65005 NZ_CP016755:c2554465-2554316 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT65005 NZ_CP016755:2554094-2554272 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin