Detailed information of TA system TA064974

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1728243..1728840
• Replicon: chromosome
• Accession: NZ_CP016673
• Organism: Neisseria meningitidis strain M25459

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0Y6JZK6
• Locus tag: A6L96_RS09060
• Protein ID: WP_002242826.1
• Coordinates: 1728562..1728840 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A6L96_RS09055
• Protein ID: WP_002221020.1
• Coordinates: 1728243..1728554 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6L96_RS13660	1724174..1724338	+	165	WP_002224559.1	baseplate J/gp47 family protein	-
A6L96_RS09040	1724338..1724949	+	612	WP_002224558.1	baseplate J/gp47 family protein	-
A6L96_RS09045	1724946..1725506	+	561	WP_002213590.1	YmfQ family protein	-
A6L96_RS09050	1725517..1727490	+	1974	WP_002248050.1	pyocin knob domain-containing protein	-
A6L96_RS09055	1728243..1728554	-	312	WP_002221020.1	HigA family addiction module antidote protein	Antitoxin
A6L96_RS09060	1728562..1728840	-	279	WP_002242826.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A6L96_RS09065	1728925..1729530	+	606	WP_002219301.1	hypothetical protein	-
A6L96_RS09070	1729509..1729805	+	297	WP_002221017.1	hypothetical protein	-
A6L96_RS09075	1729802..1730011	+	210	WP_002222487.1	hypothetical protein	-
A6L96_RS09080	1730121..1731264	-	1144	Protein_1699	ABC transporter ATP-binding protein/permease	-
A6L96_RS09095	1731706..1732353	-	648	WP_002217218.1	DUF799 domain-containing protein	-
A6L96_RS09100	1732350..1732718	-	369	WP_002219297.1	DUF4810 domain-containing protein	-
A6L96_RS09105	1732728..1733399	-	672	WP_002217215.1	CsgG/HfaB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1713947..1729805	15858
	-	flank	IS/Tn	-	-	1723144..1724109	965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (3-80)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10237.66 Da
• Isoelectric Point: 10.0980

>T64974 WP_002242826.1 NZ_CP016673:c1728840-1728562 [Neisseria meningitidis]
MIVSFKHKGLERFFKTGSLSGIQAGHSVKLNLLLTALNAAQTPSDMAVPSWNLHPLKGSLSGHWAVKVNGNWRLTFRFND
GNAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T64974 NZ_CP016673:c1728840-1728562 [Neisseria meningitidis]
ATGATTGTTTCTTTCAAACACAAAGGGCTTGAACGCTTTTTTAAAACAGGCTCGCTATCGGGCATCCAAGCCGGGCATTC
CGTCAAACTGAATCTGCTTCTAACCGCGCTGAATGCCGCGCAAACGCCTTCGGATATGGCTGTGCCGAGTTGGAATCTTC
ATCCCCTCAAGGGCAGTTTGTCGGGACATTGGGCGGTTAAAGTCAACGGAAATTGGCGTTTGACTTTTCGTTTCAACGAC
GGCAATGCCGAAGTTGTCGATTATCAGGATTATCACTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11476.32 Da
• Isoelectric Point: 10.5523

>AT64974 WP_002221020.1 NZ_CP016673:c1728554-1728243 [Neisseria meningitidis]
MKMHNPAHPGLVLKEYINGASITDIAKRLGVSRVALSRIVNAQSSITPEMAVRLSQLLNTSPDFWLNMQAGYDLWQIRQR
KTFDIAPLFPEPPLSSMQTGARA

Nucleotide

• Length: 312 bp

>AT64974 NZ_CP016673:c1728554-1728243 [Neisseria meningitidis]
ATGAAAATGCACAATCCTGCCCACCCGGGCCTTGTTTTAAAAGAGTATATTAACGGTGCAAGCATTACCGATATAGCGAA
ACGCCTGGGTGTCAGCCGGGTTGCCTTATCGCGCATTGTCAACGCCCAATCTTCGATTACGCCCGAGATGGCGGTTCGTT
TAAGCCAACTGCTCAATACTTCGCCTGATTTTTGGCTGAATATGCAGGCCGGTTATGATTTGTGGCAAATCAGGCAACGC
AAAACTTTTGATATTGCGCCCTTATTTCCCGAACCGCCTTTGTCTTCGATGCAAACAGGTGCAAGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0Y6JZK6