Detailed information of TA system TA064973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1461629..1462226
• Replicon: chromosome
• Accession: NZ_CP016670
• Organism: Neisseria meningitidis strain M25087

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0Y6JZK6
• Locus tag: A6L77_RS07450
• Protein ID: WP_002242826.1
• Coordinates: 1461629..1461907 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A6L77_RS07455
• Protein ID: WP_002221020.1
• Coordinates: 1461915..1462226 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6L77_RS07405	1457070..1457741	+	672	WP_002217215.1	CsgG/HfaB family protein	-
A6L77_RS07410	1457751..1458119	+	369	WP_002219297.1	DUF4810 domain-containing protein	-
A6L77_RS07415	1458116..1458763	+	648	WP_002217218.1	DUF799 domain-containing protein	-
A6L77_RS07430	1459205..1460348	+	1144	Protein_1368	ABC transporter ATP-binding protein/permease	-
A6L77_RS07435	1460458..1460667	-	210	WP_002222487.1	hypothetical protein	-
A6L77_RS07440	1460664..1460960	-	297	WP_002221017.1	hypothetical protein	-
A6L77_RS07445	1460939..1461544	-	606	WP_002219301.1	hypothetical protein	-
A6L77_RS07450	1461629..1461907	+	279	WP_002242826.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A6L77_RS07455	1461915..1462226	+	312	WP_002221020.1	HigA family addiction module antidote protein	Antitoxin
A6L77_RS07460	1462967..1464940	-	1974	WP_002248050.1	pyocin knob domain-containing protein	-
A6L77_RS07465	1464951..1465511	-	561	WP_002213590.1	YmfQ family protein	-
A6L77_RS07470	1465508..1466119	-	612	WP_002224558.1	baseplate J/gp47 family protein	-
A6L77_RS13535	1466119..1466283	-	165	WP_002224559.1	baseplate J/gp47 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1460664..1476510	15846
	-	flank	IS/Tn	-	-	1466348..1467313	965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (3-80)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10237.66 Da
• Isoelectric Point: 10.0980

>T64973 WP_002242826.1 NZ_CP016670:1461629-1461907 [Neisseria meningitidis]
MIVSFKHKGLERFFKTGSLSGIQAGHSVKLNLLLTALNAAQTPSDMAVPSWNLHPLKGSLSGHWAVKVNGNWRLTFRFND
GNAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T64973 NZ_CP016670:1461629-1461907 [Neisseria meningitidis]
ATGATTGTTTCTTTCAAACACAAAGGGCTTGAACGCTTTTTTAAAACAGGCTCGCTATCGGGCATCCAAGCCGGGCATTC
CGTCAAACTGAATCTGCTTCTAACCGCGCTGAATGCCGCGCAAACGCCTTCGGATATGGCTGTGCCGAGTTGGAATCTTC
ATCCCCTCAAGGGCAGTTTGTCGGGACATTGGGCGGTTAAAGTCAACGGAAATTGGCGTTTGACTTTTCGTTTCAACGAC
GGCAATGCCGAAGTTGTCGATTATCAGGATTATCACTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11476.32 Da
• Isoelectric Point: 10.5523

>AT64973 WP_002221020.1 NZ_CP016670:1461915-1462226 [Neisseria meningitidis]
MKMHNPAHPGLVLKEYINGASITDIAKRLGVSRVALSRIVNAQSSITPEMAVRLSQLLNTSPDFWLNMQAGYDLWQIRQR
KTFDIAPLFPEPPLSSMQTGARA

Nucleotide

• Length: 312 bp

>AT64973 NZ_CP016670:1461915-1462226 [Neisseria meningitidis]
ATGAAAATGCACAATCCTGCCCACCCGGGCCTTGTTTTAAAAGAGTATATTAACGGTGCAAGCATTACCGATATAGCGAA
ACGCCTGGGTGTCAGCCGGGTTGCCTTATCGCGCATTGTCAACGCCCAATCTTCGATTACGCCCGAGATGGCGGTTCGTT
TAAGCCAACTGCTCAATACTTCGCCTGATTTTTGGCTGAATATGCAGGCCGGTTATGATTTGTGGCAAATCAGGCAACGC
AAAACTTTTGATATTGCGCCCTTATTTCCCGAACCGCCTTTGTCTTCGATGCAAACAGGTGCAAGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0Y6JZK6