Detailed information of TA system TA064971

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 594355..594952
• Replicon: chromosome
• Accession: NZ_CP016668
• Organism: Neisseria meningitidis strain M25472

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0Y6JZK6
• Locus tag: A6M04_RS03260
• Protein ID: WP_002242826.1
• Coordinates: 594674..594952 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A6M04_RS03255
• Protein ID: WP_002221020.1
• Coordinates: 594355..594666 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6M04_RS13350	590298..590462	+	165	WP_002224559.1	baseplate J/gp47 family protein	-
A6M04_RS03235	590462..591073	+	612	WP_002224558.1	baseplate J/gp47 family protein	-
A6M04_RS03240	591070..591630	+	561	WP_002213590.1	YmfQ family protein	-
A6M04_RS03245	591641..593614	+	1974	WP_002248050.1	pyocin knob domain-containing protein	-
A6M04_RS03255	594355..594666	-	312	WP_002221020.1	HigA family addiction module antidote protein	Antitoxin
A6M04_RS03260	594674..594952	-	279	WP_002242826.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A6M04_RS03265	595037..595642	+	606	WP_002219301.1	hypothetical protein	-
A6M04_RS03270	595621..595917	+	297	WP_002221017.1	hypothetical protein	-
A6M04_RS03275	595914..596123	+	210	WP_002222487.1	hypothetical protein	-
A6M04_RS03280	596233..597376	-	1144	Protein_628	ABC transporter ATP-binding protein/permease	-
A6M04_RS03295	597818..598465	-	648	WP_002217218.1	DUF799 domain-containing protein	-
A6M04_RS03300	598462..598830	-	369	WP_002219297.1	DUF4810 domain-containing protein	-
A6M04_RS03305	598840..599511	-	672	WP_002217215.1	CsgG/HfaB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	582416..595917	13501
	-	flank	IS/Tn	-	-	589268..590233	965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (3-80)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10237.66 Da
• Isoelectric Point: 10.0980

>T64971 WP_002242826.1 NZ_CP016668:c594952-594674 [Neisseria meningitidis]
MIVSFKHKGLERFFKTGSLSGIQAGHSVKLNLLLTALNAAQTPSDMAVPSWNLHPLKGSLSGHWAVKVNGNWRLTFRFND
GNAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T64971 NZ_CP016668:c594952-594674 [Neisseria meningitidis]
ATGATTGTTTCTTTCAAACACAAAGGGCTTGAACGCTTTTTTAAAACAGGCTCGCTATCGGGCATCCAAGCCGGGCATTC
CGTCAAACTGAATCTGCTTCTAACCGCGCTGAATGCCGCGCAAACGCCTTCGGATATGGCTGTGCCGAGTTGGAATCTTC
ATCCCCTCAAGGGCAGTTTGTCGGGACATTGGGCGGTTAAAGTCAACGGAAATTGGCGTTTGACTTTTCGTTTCAACGAC
GGCAATGCCGAAGTTGTCGATTATCAGGATTATCACTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11476.32 Da
• Isoelectric Point: 10.5523

>AT64971 WP_002221020.1 NZ_CP016668:c594666-594355 [Neisseria meningitidis]
MKMHNPAHPGLVLKEYINGASITDIAKRLGVSRVALSRIVNAQSSITPEMAVRLSQLLNTSPDFWLNMQAGYDLWQIRQR
KTFDIAPLFPEPPLSSMQTGARA

Nucleotide

• Length: 312 bp

>AT64971 NZ_CP016668:c594666-594355 [Neisseria meningitidis]
ATGAAAATGCACAATCCTGCCCACCCGGGCCTTGTTTTAAAAGAGTATATTAACGGTGCAAGCATTACCGATATAGCGAA
ACGCCTGGGTGTCAGCCGGGTTGCCTTATCGCGCATTGTCAACGCCCAATCTTCGATTACGCCCGAGATGGCGGTTCGTT
TAAGCCAACTGCTCAATACTTCGCCTGATTTTTGGCTGAATATGCAGGCCGGTTATGATTTGTGGCAAATCAGGCAACGC
AAAACTTTTGATATTGCGCCCTTATTTCCCGAACCGCCTTTGTCTTCGATGCAAACAGGTGCAAGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0Y6JZK6