Detailed information of TA system TA064969

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1310973..1311570
• Replicon: chromosome
• Accession: NZ_CP016666
• Organism: Neisseria meningitidis strain M25462

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0Y6JZK6
• Locus tag: A6L97_RS06670
• Protein ID: WP_002242826.1
• Coordinates: 1310973..1311251 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A6L97_RS06675
• Protein ID: WP_002221020.1
• Coordinates: 1311259..1311570 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6L97_RS06625	1306414..1307085	+	672	WP_002217215.1	CsgG/HfaB family protein	-
A6L97_RS06630	1307095..1307463	+	369	WP_002219297.1	DUF4810 domain-containing protein	-
A6L97_RS06635	1307460..1308107	+	648	WP_002217218.1	DUF799 domain-containing protein	-
A6L97_RS06650	1308549..1309692	+	1144	Protein_1220	ABC transporter ATP-binding protein/permease	-
A6L97_RS06655	1309802..1310011	-	210	WP_002222487.1	hypothetical protein	-
A6L97_RS06660	1310008..1310304	-	297	WP_002221017.1	hypothetical protein	-
A6L97_RS06665	1310283..1310888	-	606	WP_002219301.1	hypothetical protein	-
A6L97_RS06670	1310973..1311251	+	279	WP_002242826.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A6L97_RS06675	1311259..1311570	+	312	WP_002221020.1	HigA family addiction module antidote protein	Antitoxin
A6L97_RS06680	1312323..1314296	-	1974	WP_002248050.1	pyocin knob domain-containing protein	-
A6L97_RS06685	1314307..1314867	-	561	WP_002213590.1	YmfQ family protein	-
A6L97_RS06690	1314864..1315475	-	612	WP_002224558.1	baseplate J/gp47 family protein	-
A6L97_RS13480	1315475..1315639	-	165	WP_002224559.1	baseplate J/gp47 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1310008..1325866	15858
	-	flank	IS/Tn	-	-	1315704..1316669	965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (3-80)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10237.66 Da
• Isoelectric Point: 10.0980

>T64969 WP_002242826.1 NZ_CP016666:1310973-1311251 [Neisseria meningitidis]
MIVSFKHKGLERFFKTGSLSGIQAGHSVKLNLLLTALNAAQTPSDMAVPSWNLHPLKGSLSGHWAVKVNGNWRLTFRFND
GNAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T64969 NZ_CP016666:1310973-1311251 [Neisseria meningitidis]
ATGATTGTTTCTTTCAAACACAAAGGGCTTGAACGCTTTTTTAAAACAGGCTCGCTATCGGGCATCCAAGCCGGGCATTC
CGTCAAACTGAATCTGCTTCTAACCGCGCTGAATGCCGCGCAAACGCCTTCGGATATGGCTGTGCCGAGTTGGAATCTTC
ATCCCCTCAAGGGCAGTTTGTCGGGACATTGGGCGGTTAAAGTCAACGGAAATTGGCGTTTGACTTTTCGTTTCAACGAC
GGCAATGCCGAAGTTGTCGATTATCAGGATTATCACTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11476.32 Da
• Isoelectric Point: 10.5523

>AT64969 WP_002221020.1 NZ_CP016666:1311259-1311570 [Neisseria meningitidis]
MKMHNPAHPGLVLKEYINGASITDIAKRLGVSRVALSRIVNAQSSITPEMAVRLSQLLNTSPDFWLNMQAGYDLWQIRQR
KTFDIAPLFPEPPLSSMQTGARA

Nucleotide

• Length: 312 bp

>AT64969 NZ_CP016666:1311259-1311570 [Neisseria meningitidis]
ATGAAAATGCACAATCCTGCCCACCCGGGCCTTGTTTTAAAAGAGTATATTAACGGTGCAAGCATTACCGATATAGCGAA
ACGCCTGGGTGTCAGCCGGGTTGCCTTATCGCGCATTGTCAACGCCCAATCTTCGATTACGCCCGAGATGGCGGTTCGTT
TAAGCCAACTGCTCAATACTTCGCCTGATTTTTGGCTGAATATGCAGGCCGGTTATGATTTGTGGCAAATCAGGCAACGC
AAAACTTTTGATATTGCGCCCTTATTTCCCGAACCGCCTTTGTCTTCGATGCAAACAGGTGCAAGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0Y6JZK6