Detailed information of TA system TA064967

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1997704..1998301
• Replicon: chromosome
• Accession: NZ_CP016664
• Organism: Neisseria meningitidis strain M25070

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0Y6JZK6
• Locus tag: A6L64_RS10340
• Protein ID: WP_002242826.1
• Coordinates: 1998023..1998301 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A6L64_RS10335
• Protein ID: WP_002221020.1
• Coordinates: 1997704..1998015 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6L64_RS13765	1993647..1993811	+	165	WP_002224559.1	baseplate J/gp47 family protein	-
A6L64_RS10315	1993811..1994422	+	612	WP_002224558.1	baseplate J/gp47 family protein	-
A6L64_RS10320	1994419..1994979	+	561	WP_002213590.1	YmfQ family protein	-
A6L64_RS10325	1994990..1996963	+	1974	WP_002248050.1	pyocin knob domain-containing protein	-
A6L64_RS10335	1997704..1998015	-	312	WP_002221020.1	HigA family addiction module antidote protein	Antitoxin
A6L64_RS10340	1998023..1998301	-	279	WP_002242826.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A6L64_RS10345	1998386..1998991	+	606	WP_002219301.1	hypothetical protein	-
A6L64_RS10350	1998970..1999266	+	297	WP_002221017.1	hypothetical protein	-
A6L64_RS10355	1999263..1999472	+	210	WP_002222487.1	hypothetical protein	-
A6L64_RS10360	1999582..2000725	-	1144	Protein_1934	ABC transporter ATP-binding protein/permease	-
A6L64_RS10375	2001167..2001814	-	648	WP_002217218.1	DUF799 domain-containing protein	-
A6L64_RS10380	2001811..2002179	-	369	WP_002219297.1	DUF4810 domain-containing protein	-
A6L64_RS10385	2002189..2002860	-	672	WP_002217215.1	CsgG/HfaB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1983420..1999266	15846
	-	flank	IS/Tn	-	-	1992617..1993582	965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (3-80)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10237.66 Da
• Isoelectric Point: 10.0980

>T64967 WP_002242826.1 NZ_CP016664:c1998301-1998023 [Neisseria meningitidis]
MIVSFKHKGLERFFKTGSLSGIQAGHSVKLNLLLTALNAAQTPSDMAVPSWNLHPLKGSLSGHWAVKVNGNWRLTFRFND
GNAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T64967 NZ_CP016664:c1998301-1998023 [Neisseria meningitidis]
ATGATTGTTTCTTTCAAACACAAAGGGCTTGAACGCTTTTTTAAAACAGGCTCGCTATCGGGCATCCAAGCCGGGCATTC
CGTCAAACTGAATCTGCTTCTAACCGCGCTGAATGCCGCGCAAACGCCTTCGGATATGGCTGTGCCGAGTTGGAATCTTC
ATCCCCTCAAGGGCAGTTTGTCGGGACATTGGGCGGTTAAAGTCAACGGAAATTGGCGTTTGACTTTTCGTTTCAACGAC
GGCAATGCCGAAGTTGTCGATTATCAGGATTATCACTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11476.32 Da
• Isoelectric Point: 10.5523

>AT64967 WP_002221020.1 NZ_CP016664:c1998015-1997704 [Neisseria meningitidis]
MKMHNPAHPGLVLKEYINGASITDIAKRLGVSRVALSRIVNAQSSITPEMAVRLSQLLNTSPDFWLNMQAGYDLWQIRQR
KTFDIAPLFPEPPLSSMQTGARA

Nucleotide

• Length: 312 bp

>AT64967 NZ_CP016664:c1998015-1997704 [Neisseria meningitidis]
ATGAAAATGCACAATCCTGCCCACCCGGGCCTTGTTTTAAAAGAGTATATTAACGGTGCAAGCATTACCGATATAGCGAA
ACGCCTGGGTGTCAGCCGGGTTGCCTTATCGCGCATTGTCAACGCCCAATCTTCGATTACGCCCGAGATGGCGGTTCGTT
TAAGCCAACTGCTCAATACTTCGCCTGATTTTTGGCTGAATATGCAGGCCGGTTATGATTTGTGGCAAATCAGGCAACGC
AAAACTTTTGATATTGCGCCCTTATTTCCCGAACCGCCTTTGTCTTCGATGCAAACAGGTGCAAGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0Y6JZK6