Detailed information of TA system TA064965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1994955..1995552
• Replicon: chromosome
• Accession: NZ_CP016662
• Organism: Neisseria meningitidis strain M23413

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0Y6JZK6
• Locus tag: A6L52_RS10260
• Protein ID: WP_002242826.1
• Coordinates: 1994955..1995233 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A6L52_RS10265
• Protein ID: WP_079870528.1
• Coordinates: 1995241..1995552 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6L52_RS10215	1990396..1991067	+	672	WP_002217215.1	CsgG/HfaB family protein	-
A6L52_RS10220	1991077..1991445	+	369	WP_002219297.1	DUF4810 domain-containing protein	-
A6L52_RS10225	1991442..1992089	+	648	WP_002217218.1	DUF799 domain-containing protein	-
A6L52_RS10240	1992531..1993674	+	1144	Protein_1903	ABC transporter ATP-binding protein/permease	-
A6L52_RS10245	1993784..1993993	-	210	WP_002222487.1	hypothetical protein	-
A6L52_RS10250	1993990..1994286	-	297	WP_002221017.1	hypothetical protein	-
A6L52_RS10255	1994265..1994870	-	606	WP_002219301.1	hypothetical protein	-
A6L52_RS10260	1994955..1995233	+	279	WP_002242826.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A6L52_RS10265	1995241..1995552	+	312	WP_079870528.1	HigA family addiction module antidote protein	Antitoxin
A6L52_RS10270	1996293..1998266	-	1974	WP_002248050.1	pyocin knob domain-containing protein	-
A6L52_RS10275	1998277..1998837	-	561	WP_002213590.1	YmfQ family protein	-
A6L52_RS10280	1998834..1999445	-	612	WP_002224558.1	baseplate J/gp47 family protein	-
A6L52_RS13760	1999445..1999609	-	165	WP_002224559.1	baseplate J/gp47 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1993990..2007491	13501
	-	flank	IS/Tn	-	-	1999674..2000639	965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-81)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10237.66 Da
• Isoelectric Point: 10.0980

>T64965 WP_002242826.1 NZ_CP016662:1994955-1995233 [Neisseria meningitidis]
MIVSFKHKGLERFFKTGSLSGIQAGHSVKLNLLLTALNAAQTPSDMAVPSWNLHPLKGSLSGHWAVKVNGNWRLTFRFND
GNAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T64965 NZ_CP016662:1994955-1995233 [Neisseria meningitidis]
ATGATTGTTTCTTTCAAACACAAAGGGCTTGAACGCTTTTTTAAAACAGGCTCGCTATCGGGCATCCAAGCCGGGCATTC
CGTCAAACTGAATCTGCTTCTAACCGCGCTGAATGCCGCGCAAACGCCTTCGGATATGGCTGTGCCGAGTTGGAATCTTC
ATCCCCTCAAGGGCAGTTTGTCGGGACATTGGGCGGTTAAAGTCAACGGAAATTGGCGTTTGACTTTTCGTTTCAACGAC
GGCAATGCCGAAGTTGTCGATTATCAGGATTATCACTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11433.30 Da
• Isoelectric Point: 10.2202

>AT64965 WP_079870528.1 NZ_CP016662:1995241-1995552 [Neisseria meningitidis]
MKMHNPAHPGLVLKEYINGASITDIAKRLGVSLVALSRIVNAQSSITPEMAVRLSQLLNTSPDFWLNMQAGYDLWQIRQR
KTFDIAPLFPEPPLSSMQTGARA

Nucleotide

• Length: 312 bp

>AT64965 NZ_CP016662:1995241-1995552 [Neisseria meningitidis]
ATGAAAATGCACAATCCTGCCCACCCGGGCCTTGTTTTAAAAGAGTATATTAACGGTGCAAGCATTACCGATATAGCGAA
ACGCCTGGGTGTCAGCCTGGTTGCCTTATCGCGCATTGTCAACGCCCAATCTTCGATTACGCCCGAGATGGCGGTTCGTT
TAAGCCAACTGCTCAATACTTCGCCTGATTTTTGGCTGAATATGCAGGCCGGTTATGATTTGTGGCAAATCAGGCAACGC
AAAACTTTTGATATTGCGCCCTTATTTCCCGAACCGCCTTTGTCTTCGATGCAAACAGGTGCAAGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0Y6JZK6