Detailed information of TA system TA064963

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 821949..822546
• Replicon: chromosome
• Accession: NZ_CP016659
• Organism: Neisseria meningitidis strain M22801

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0Y6JZK6
• Locus tag: A6L45_RS04390
• Protein ID: WP_002242826.1
• Coordinates: 822268..822546 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A6L45_RS04385
• Protein ID: WP_002221020.1
• Coordinates: 821949..822260 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6L45_RS13525	817867..818031	+	165	WP_002224559.1	baseplate J/gp47 family protein	-
A6L45_RS04370	818031..818642	+	612	WP_002224558.1	baseplate J/gp47 family protein	-
A6L45_RS04375	818639..819199	+	561	WP_002213590.1	YmfQ family protein	-
A6L45_RS04380	819210..821183	+	1974	WP_002248050.1	pyocin knob domain-containing protein	-
A6L45_RS04385	821949..822260	-	312	WP_002221020.1	HigA family addiction module antidote protein	Antitoxin
A6L45_RS04390	822268..822546	-	279	WP_002242826.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A6L45_RS04395	822631..823236	+	606	WP_002219301.1	hypothetical protein	-
A6L45_RS04400	823215..823511	+	297	WP_002221017.1	hypothetical protein	-
A6L45_RS04405	823508..823717	+	210	WP_002222487.1	hypothetical protein	-
A6L45_RS04410	823827..824970	-	1144	Protein_848	ABC transporter ATP-binding protein/permease	-
A6L45_RS04425	825412..826059	-	648	WP_002217218.1	DUF799 domain-containing protein	-
A6L45_RS04430	826056..826424	-	369	WP_002219297.1	DUF4810 domain-containing protein	-
A6L45_RS04435	826434..827105	-	672	WP_002217215.1	CsgG/HfaB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	807640..823511	15871
	-	flank	IS/Tn	-	-	816837..817802	965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (3-80)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10237.66 Da
• Isoelectric Point: 10.0980

>T64963 WP_002242826.1 NZ_CP016659:c822546-822268 [Neisseria meningitidis]
MIVSFKHKGLERFFKTGSLSGIQAGHSVKLNLLLTALNAAQTPSDMAVPSWNLHPLKGSLSGHWAVKVNGNWRLTFRFND
GNAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T64963 NZ_CP016659:c822546-822268 [Neisseria meningitidis]
ATGATTGTTTCTTTCAAACACAAAGGGCTTGAACGCTTTTTTAAAACAGGCTCGCTATCGGGCATCCAAGCCGGGCATTC
CGTCAAACTGAATCTGCTTCTAACCGCGCTGAATGCCGCGCAAACGCCTTCGGATATGGCTGTGCCGAGTTGGAATCTTC
ATCCCCTCAAGGGCAGTTTGTCGGGACATTGGGCGGTTAAAGTCAACGGAAATTGGCGTTTGACTTTTCGTTTCAACGAC
GGCAATGCCGAAGTTGTCGATTATCAGGATTATCACTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11476.32 Da
• Isoelectric Point: 10.5523

>AT64963 WP_002221020.1 NZ_CP016659:c822260-821949 [Neisseria meningitidis]
MKMHNPAHPGLVLKEYINGASITDIAKRLGVSRVALSRIVNAQSSITPEMAVRLSQLLNTSPDFWLNMQAGYDLWQIRQR
KTFDIAPLFPEPPLSSMQTGARA

Nucleotide

• Length: 312 bp

>AT64963 NZ_CP016659:c822260-821949 [Neisseria meningitidis]
ATGAAAATGCACAATCCTGCCCACCCGGGCCTTGTTTTAAAAGAGTATATTAACGGTGCAAGCATTACCGATATAGCGAA
ACGCCTGGGTGTCAGCCGGGTTGCCTTATCGCGCATTGTCAACGCCCAATCTTCGATTACGCCCGAGATGGCGGTTCGTT
TAAGCCAACTGCTCAATACTTCGCCTGATTTTTGGCTGAATATGCAGGCCGGTTATGATTTGTGGCAAATCAGGCAACGC
AAAACTTTTGATATTGCGCCCTTATTTCCCGAACCGCCTTTGTCTTCGATGCAAACAGGTGCAAGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0Y6JZK6