Detailed information of TA system TA064956

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1290215..1290812
• Replicon: chromosome
• Accession: NZ_CP016651
• Organism: Neisseria meningitidis strain M25474

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0Y6JZK6
• Locus tag: A6M06_RS06545
• Protein ID: WP_002242826.1
• Coordinates: 1290215..1290493 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A6M06_RS06550
• Protein ID: WP_002221020.1
• Coordinates: 1290501..1290812 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6M06_RS06500	1285656..1286327	+	672	WP_002217215.1	CsgG/HfaB family protein	-
A6M06_RS06505	1286337..1286705	+	369	WP_002219297.1	DUF4810 domain-containing protein	-
A6M06_RS06510	1286702..1287349	+	648	WP_002217218.1	DUF799 domain-containing protein	-
A6M06_RS06525	1287791..1288934	+	1144	Protein_1198	ABC transporter ATP-binding protein/permease	-
A6M06_RS06530	1289044..1289253	-	210	WP_002222487.1	hypothetical protein	-
A6M06_RS06535	1289250..1289546	-	297	WP_002221017.1	hypothetical protein	-
A6M06_RS06540	1289525..1290130	-	606	WP_002219301.1	hypothetical protein	-
A6M06_RS06545	1290215..1290493	+	279	WP_002242826.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A6M06_RS06550	1290501..1290812	+	312	WP_002221020.1	HigA family addiction module antidote protein	Antitoxin
A6M06_RS06555	1291553..1293526	-	1974	WP_002248050.1	pyocin knob domain-containing protein	-
A6M06_RS06560	1293537..1294097	-	561	WP_002213590.1	YmfQ family protein	-
A6M06_RS06565	1294094..1294705	-	612	WP_002224558.1	baseplate J/gp47 family protein	-
A6M06_RS13470	1294705..1294869	-	165	WP_002224559.1	baseplate J/gp47 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1289250..1305096	15846
	-	flank	IS/Tn	-	-	1294934..1295899	965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (3-80)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10237.66 Da
• Isoelectric Point: 10.0980

>T64956 WP_002242826.1 NZ_CP016651:1290215-1290493 [Neisseria meningitidis]
MIVSFKHKGLERFFKTGSLSGIQAGHSVKLNLLLTALNAAQTPSDMAVPSWNLHPLKGSLSGHWAVKVNGNWRLTFRFND
GNAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T64956 NZ_CP016651:1290215-1290493 [Neisseria meningitidis]
ATGATTGTTTCTTTCAAACACAAAGGGCTTGAACGCTTTTTTAAAACAGGCTCGCTATCGGGCATCCAAGCCGGGCATTC
CGTCAAACTGAATCTGCTTCTAACCGCGCTGAATGCCGCGCAAACGCCTTCGGATATGGCTGTGCCGAGTTGGAATCTTC
ATCCCCTCAAGGGCAGTTTGTCGGGACATTGGGCGGTTAAAGTCAACGGAAATTGGCGTTTGACTTTTCGTTTCAACGAC
GGCAATGCCGAAGTTGTCGATTATCAGGATTATCACTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11476.32 Da
• Isoelectric Point: 10.5523

>AT64956 WP_002221020.1 NZ_CP016651:1290501-1290812 [Neisseria meningitidis]
MKMHNPAHPGLVLKEYINGASITDIAKRLGVSRVALSRIVNAQSSITPEMAVRLSQLLNTSPDFWLNMQAGYDLWQIRQR
KTFDIAPLFPEPPLSSMQTGARA

Nucleotide

• Length: 312 bp

>AT64956 NZ_CP016651:1290501-1290812 [Neisseria meningitidis]
ATGAAAATGCACAATCCTGCCCACCCGGGCCTTGTTTTAAAAGAGTATATTAACGGTGCAAGCATTACCGATATAGCGAA
ACGCCTGGGTGTCAGCCGGGTTGCCTTATCGCGCATTGTCAACGCCCAATCTTCGATTACGCCCGAGATGGCGGTTCGTT
TAAGCCAACTGCTCAATACTTCGCCTGATTTTTGGCTGAATATGCAGGCCGGTTATGATTTGTGGCAAATCAGGCAACGC
AAAACTTTTGATATTGCGCCCTTATTTCCCGAACCGCCTTTGTCTTCGATGCAAACAGGTGCAAGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0Y6JZK6