Detailed information of TA system TA064939

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4665296..4665898
• Replicon: chromosome
• Accession: NZ_CP016628
• Organism: Escherichia coli strain FORC_041

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: FORC41_RS24295
• Protein ID: WP_000897305.1
• Coordinates: 4665587..4665898 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FORC41_RS24290
• Protein ID: WP_000356397.1
• Coordinates: 4665296..4665586 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC41_RS24260	4661222..4662124	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
FORC41_RS24265	4662121..4662756	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
FORC41_RS24270	4662753..4663682	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
FORC41_RS24280	4664012..4664254	-	243	WP_001086388.1	hypothetical protein	-
FORC41_RS24285	4664473..4664691	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
FORC41_RS24290	4665296..4665586	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
FORC41_RS24295	4665587..4665898	-	312	WP_000897305.1	hypothetical protein	Toxin
FORC41_RS24300	4666127..4667035	+	909	WP_001162704.1	alpha/beta hydrolase	-
FORC41_RS24305	4667099..4668040	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
FORC41_RS24310	4668085..4668522	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FORC41_RS24315	4668519..4669391	-	873	WP_000920762.1	virulence factor BrkB family protein	-
FORC41_RS24320	4669385..4669984	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T64939 WP_000897305.1 NZ_CP016628:c4665898-4665587 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T64939 NZ_CP016628:c4665898-4665587 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT64939 WP_000356397.1 NZ_CP016628:c4665586-4665296 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT64939 NZ_CP016628:c4665586-4665296 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7