Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3842188..3842729 Replicon chromosome
Accession NZ_CP016628
Organism Escherichia coli strain FORC_041

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag FORC41_RS20115 Protein ID WP_000615976.1
Coordinates 3842451..3842729 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag FORC41_RS20110 Protein ID WP_000729704.1
Coordinates 3842188..3842448 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC41_RS20085 3837971..3838756 - 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -
FORC41_RS20090 3838728..3840440 + 1713 Protein_3649 flagellar biosynthesis protein FlhA -
FORC41_RS20095 3840545..3840823 + 279 WP_047081734.1 type II toxin-antitoxin system HicA family toxin -
FORC41_RS20100 3840816..3841172 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
FORC41_RS20105 3841229..3842002 - 774 WP_000543895.1 C40 family peptidase -
FORC41_RS20110 3842188..3842448 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
FORC41_RS20115 3842451..3842729 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
FORC41_RS20120 3842885..3843625 + 741 WP_001225679.1 murein L,D-transpeptidase -
FORC41_RS20125 3843596..3844363 - 768 WP_000333380.1 class II glutamine amidotransferase -
FORC41_RS20135 3844569..3845147 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3840816..3852342 11526


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T64936 WP_000615976.1 NZ_CP016628:3842451-3842729 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T64936 NZ_CP016628:3842451-3842729 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT64936 WP_000729704.1 NZ_CP016628:3842188-3842448 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT64936 NZ_CP016628:3842188-3842448 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References