Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1910235..1910737 Replicon chromosome
Accession NZ_CP016628
Organism Escherichia coli strain FORC_041

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FORC41_RS09855 Protein ID WP_000767829.1
Coordinates 1910483..1910737 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FORC41_RS09850 Protein ID WP_001259255.1
Coordinates 1910235..1910486 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC41_RS09820 1905413..1906480 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
FORC41_RS09825 1906480..1907550 - 1071 WP_021578586.1 histidinol-phosphate transaminase -
FORC41_RS09830 1907547..1908851 - 1305 WP_032251908.1 histidinol dehydrogenase -
FORC41_RS09835 1908857..1909756 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
FORC41_RS09845 1909902..1909952 - 51 WP_001364200.1 his operon leader peptide -
FORC41_RS09850 1910235..1910486 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FORC41_RS09855 1910483..1910737 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FORC41_RS09860 1910820..1911644 + 825 WP_000754758.1 SDR family oxidoreductase -
FORC41_RS09865 1911690..1912619 + 930 WP_000803351.1 LysR family transcriptional regulator -
FORC41_RS26135 1912834..1912896 + 63 WP_010723108.1 membrane protein YoeI -
FORC41_RS09870 1912886..1914244 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FORC41_RS09875 1914423..1915481 + 1059 WP_024187793.1 transport protein YeeE -
FORC41_RS09880 1915495..1915722 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T64924 WP_000767829.1 NZ_CP016628:1910483-1910737 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T64924 NZ_CP016628:1910483-1910737 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT64924 WP_001259255.1 NZ_CP016628:1910235-1910486 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT64924 NZ_CP016628:1910235-1910486 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References