Detailed information of TA system TA064886

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2285350..2285575
• Replicon: chromosome
• Accession: NZ_CP016625
• Organism: Escherichia coli O157:H7 strain FRIK944

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: A9L45_RS11795
• Protein ID: WP_000813258.1
• Coordinates: 2285350..2285505 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2285517..2285575 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9L45_RS11745	2280353..2280784	-	432	WP_001302123.1	tellurite resistance protein	-
A9L45_RS11760	2281235..2281948	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
A9L45_RS34650	2282084..2282281	-	198	WP_000917763.1	hypothetical protein	-
A9L45_RS11770	2282506..2283060	-	555	WP_000640035.1	DUF1133 family protein	-
A9L45_RS11775	2283123..2283428	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
A9L45_RS11780	2283441..2284490	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
A9L45_RS11785	2284492..2284764	-	273	WP_000191872.1	hypothetical protein	-
A9L45_RS11790	2284886..2285230	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
A9L45_RS11795	2285350..2285505	-	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2285517..2285575	+	59	-	-	Antitoxin
A9L45_RS11800	2285796..2286353	-	558	WP_000104474.1	DUF551 domain-containing protein	-
A9L45_RS11805	2286355..2286573	-	219	WP_000683609.1	DUF4014 family protein	-
A9L45_RS11810	2286701..2287012	-	312	WP_001289673.1	hypothetical protein	-
A9L45_RS11815	2287005..2287232	-	228	WP_000699809.1	hypothetical protein	-
A9L45_RS11820	2287229..2287510	-	282	WP_000603384.1	DNA-binding protein	-
A9L45_RS11825	2287543..2288259	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
A9L45_RS34655	2288293..2288754	-	462	WP_000139447.1	replication protein	-
A9L45_RS34660	2288747..2289790	-	1044	WP_001262402.1	hypothetical protein	-
A9L45_RS11840	2289859..2290284	-	426	WP_000693878.1	Rha family transcriptional regulator	-
A9L45_RS11845	2290268..2290510	-	243	WP_000747948.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ospG	2177165..2337493	160328

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T64886 WP_000813258.1 NZ_CP016625:c2285505-2285350 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T64886 NZ_CP016625:c2285505-2285350 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT64886 NZ_CP016625:2285517-2285575 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin