Detailed information of TA system TA064884

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2213032..2213257
• Replicon: chromosome
• Accession: NZ_CP016625
• Organism: Escherichia coli O157:H7 strain FRIK944

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: A9L45_RS11365
• Protein ID: WP_000813254.1
• Coordinates: 2213032..2213187 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2213199..2213257 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9L45_RS11335	2208794..2208991	-	198	WP_000917735.1	hypothetical protein	-
A9L45_RS11340	2209218..2210039	-	822	WP_000762902.1	antitermination protein	-
A9L45_RS11345	2210036..2210410	-	375	WP_000904171.1	RusA family crossover junction endodeoxyribonuclease	-
A9L45_RS11350	2210423..2211472	-	1050	WP_001265189.1	DUF968 domain-containing protein	-
A9L45_RS31380	2211474..2211743	-	270	WP_024177817.1	hypothetical protein	-
A9L45_RS31385	2211797..2212024	-	228	WP_001452497.1	hypothetical protein	-
A9L45_RS11355	2212248..2212619	+	372	WP_001217944.1	type II toxin-antitoxin system RelE/ParE family toxin	-
A9L45_RS11360	2212612..2212929	+	318	WP_000042395.1	DNA-binding transcriptional regulator	-
A9L45_RS11365	2213032..2213187	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	2213199..2213257	+	59	-	-	Antitoxin
A9L45_RS11370	2213459..2214007	-	549	WP_000211435.1	ORF6N domain-containing protein	-
A9L45_RS11375	2214355..2214540	-	186	WP_000215514.1	hypothetical protein	-
A9L45_RS11380	2214600..2215358	-	759	WP_000537579.1	DUF1627 domain-containing protein	-
A9L45_RS34600	2215393..2215935	-	543	WP_157837342.1	replication protein	-
A9L45_RS34605	2215847..2216887	-	1041	WP_000020565.1	DNA-binding protein	-
A9L45_RS11395	2216859..2217410	-	552	WP_000705370.1	hypothetical protein	-
A9L45_RS11400	2217394..2217621	-	228	WP_000476986.1	transcriptional regulator	-
A9L45_RS11405	2217699..2218106	+	408	WP_001003379.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ospG	2177165..2337493	160328

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T64884 WP_000813254.1 NZ_CP016625:c2213187-2213032 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T64884 NZ_CP016625:c2213187-2213032 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATTGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT64884 NZ_CP016625:2213199-2213257 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGCAAAGCATGAAATTGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin