Detailed information of TA system TA064874

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1306031..1306256
• Replicon: chromosome
• Accession: NZ_CP016625
• Organism: Escherichia coli O157:H7 strain FRIK944

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: A9L45_RS06380
• Protein ID: WP_000813263.1
• Coordinates: 1306101..1306256 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1306031..1306089 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9L45_RS06355	1301306..1302397	+	1092	WP_001205823.1	hypothetical protein	-
A9L45_RS06360	1302404..1303150	+	747	WP_000788751.1	ATP-binding protein	-
A9L45_RS06365	1303172..1303942	+	771	WP_000450992.1	DUF1627 domain-containing protein	-
A9L45_RS06370	1303958..1304371	+	414	WP_001151233.1	DUF977 family protein	-
A9L45_RS06375	1304723..1305496	-	774	WP_000160654.1	alpha/beta hydrolase	-
-	1306031..1306089	-	59	-	-	Antitoxin
A9L45_RS06380	1306101..1306256	+	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
A9L45_RS33235	1306424..1306702	+	279	WP_001341388.1	hypothetical protein	-
A9L45_RS06390	1306704..1307753	+	1050	WP_001265172.1	DUF968 domain-containing protein	-
A9L45_RS06395	1307766..1308137	+	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
A9L45_RS06400	1308127..1308498	+	372	WP_000090264.1	antitermination protein	-
A9L45_RS06405	1308650..1309468	+	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
A9L45_RS06410	1309755..1309951	+	197	Protein_1218	TrmB family transcriptional regulator	-
A9L45_RS06415	1310089..1310802	+	714	WP_000261909.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T64874 WP_000813263.1 NZ_CP016625:1306101-1306256 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T64874 NZ_CP016625:1306101-1306256 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT64874 NZ_CP016625:c1306089-1306031 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin