Detailed information of TA system TA064813

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 1814799..1815451
• Replicon: plasmid plas1
• Accession: NZ_CP016555
• Organism: Ralstonia solanacearum FJAT-1458

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A454TTN3
• Locus tag: BCR16_RS26295
• Protein ID: WP_038962847.1
• Coordinates: 1814799..1815143 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BCR16_RS26300
• Protein ID: WP_020425351.1
• Coordinates: 1815140..1815451 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCR16_RS26280	1810495..1811658	-	1164	WP_016725329.1	2-methylcitrate synthase	-
BCR16_RS26285	1811710..1812606	-	897	WP_038962849.1	methylisocitrate lyase	-
BCR16_RS26290	1812799..1814748	+	1950	WP_023470006.1	propionate catabolism operon regulatory protein PrpR	-
BCR16_RS26295	1814799..1815143	+	345	WP_038962847.1	hypothetical protein	Toxin
BCR16_RS26300	1815140..1815451	+	312	WP_020425351.1	transcriptional regulator	Antitoxin
BCR16_RS26305	1815591..1816400	-	810	WP_043885575.1	DUF2242 domain-containing protein	-
BCR16_RS26310	1817792..1818778	-	987	WP_016721870.1	IS5 family transposase	-
BCR16_RS28570	1818834..1820159	+	1326	WP_016725321.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	flgC / flgG / fliM / fliG / fliI / acrB / adeG / cheY / cheB / cheW / motA / cyaB	1..2075659	2075659

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13227.94 Da
• Isoelectric Point: 8.4489

>T64813 WP_038962847.1 NZ_CP016555:1814799-1815143 [Ralstonia solanacearum FJAT-1458]
MDATFIELPPFQRLREHYLSDDEYRALQQELLARPDAGDVIKGTGGLRKLRFSDKRRGKGKRGGLRVIDYHWDGGGQFWM
FVVYDKGEADDLSSDERKVLARLLEQEVKARSER

Nucleotide

• Length: 345 bp

>T64813 NZ_CP016555:1814799-1815143 [Ralstonia solanacearum FJAT-1458]
ATGGATGCTACCTTTATCGAGTTGCCGCCTTTTCAGAGGCTGCGGGAGCACTATCTCTCGGACGACGAGTACCGAGCGCT
CCAACAGGAACTGCTGGCCCGGCCGGACGCCGGCGACGTCATCAAGGGGACAGGTGGCCTACGCAAGCTGCGATTTTCGG
ACAAGCGGCGCGGCAAAGGTAAGCGCGGCGGTCTGCGCGTGATTGACTACCACTGGGATGGAGGCGGTCAATTCTGGATG
TTTGTCGTCTACGACAAGGGCGAGGCGGACGACCTTTCCAGCGACGAGCGCAAAGTGCTGGCACGGCTGTTGGAGCAGGA
AGTCAAGGCAAGGAGTGAGCGATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11619.65 Da
• Isoelectric Point: 10.7132

>AT64813 WP_020425351.1 NZ_CP016555:1815140-1815451 [Ralstonia solanacearum FJAT-1458]
MKRNLFAELKEGMDALAAEREGKMTLRKVKVRVLQPLDVTAEEIKAVRAAAHASQAVMARRLRVNVRTYQNWEQGTAKPN
TQAAVLIKLVEKHPETLQMLEAL

Nucleotide

• Length: 312 bp

>AT64813 NZ_CP016555:1815140-1815451 [Ralstonia solanacearum FJAT-1458]
ATGAAACGGAACCTGTTTGCTGAGTTGAAGGAGGGGATGGATGCGCTTGCCGCAGAGCGCGAAGGAAAGATGACCCTTCG
CAAGGTGAAGGTCCGCGTGCTCCAGCCGCTTGACGTGACAGCCGAAGAAATCAAGGCCGTTCGCGCAGCGGCACATGCTT
CACAGGCTGTCATGGCACGTCGACTGCGCGTCAATGTGCGCACCTACCAGAACTGGGAACAAGGAACCGCCAAACCGAAC
ACCCAGGCGGCTGTGTTGATCAAGCTCGTCGAGAAGCATCCGGAAACGTTGCAGATGCTGGAAGCGCTTTAA

Structures

Toxin

• AlphaFold DB: A0A454TTN3