Detailed information of TA system TA064773

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2202618..2203220
• Replicon: chromosome
• Accession: NZ_CP016546
• Organism: Escherichia coli strain O177:H21

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: BB405_RS11060
• Protein ID: WP_000897305.1
• Coordinates: 2202909..2203220 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BB405_RS11055
• Protein ID: WP_000356397.1
• Coordinates: 2202618..2202908 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB405_RS11020	2198563..2199465	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
BB405_RS11025	2199462..2200097	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
BB405_RS11030	2200094..2201023	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
BB405_RS11040	2201353..2201595	-	243	WP_001087409.1	hypothetical protein	-
BB405_RS11045	2201814..2202032	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
BB405_RS11055	2202618..2202908	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
BB405_RS11060	2202909..2203220	-	312	WP_000897305.1	hypothetical protein	Toxin
BB405_RS11065	2203449..2204357	+	909	WP_001162704.1	alpha/beta hydrolase	-
BB405_RS11070	2204421..2205362	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
BB405_RS11075	2205407..2205844	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
BB405_RS11080	2205841..2206713	-	873	WP_000920762.1	virulence factor BrkB family protein	-
BB405_RS11085	2206707..2207306	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T64773 WP_000897305.1 NZ_CP016546:c2203220-2202909 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T64773 NZ_CP016546:c2203220-2202909 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT64773 WP_000356397.1 NZ_CP016546:c2202908-2202618 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT64773 NZ_CP016546:c2202908-2202618 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7