Detailed information of TA system TA064731

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 122362..122788
• Replicon: plasmid unnamed1
• Accession: NZ_CP016498
• Organism: Escherichia coli strain UPEC 26-1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: BB344_RS26235
• Protein ID: WP_001312861.1
• Coordinates: 122362..122520 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	122564..122788 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB344_RS26195	117760..118449	-	690	WP_000283387.1	conjugal transfer transcriptional regulator TraJ	-
BB344_RS26200	118636..119019	-	384	WP_001151538.1	relaxosome protein TraM	-
BB344_RS26205	119340..119942	+	603	Protein_131	transglycosylase SLT domain-containing protein	-
BB344_RS26210	120238..121059	-	822	WP_001234445.1	DUF945 domain-containing protein	-
BB344_RS26215	121169..121465	-	297	WP_001545326.1	hypothetical protein	-
BB344_RS30420	121857..122061	+	205	Protein_134	pilus protein	-
BB344_RS26235	122362..122520	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	122564..122788	-	225	NuclAT_0	-	Antitoxin
-	122564..122788	-	225	NuclAT_0	-	Antitoxin
-	122564..122788	-	225	NuclAT_0	-	Antitoxin
-	122564..122788	-	225	NuclAT_0	-	Antitoxin
BB344_RS30425	122600..122779	+	180	WP_001309233.1	hypothetical protein	-
BB344_RS26245	122800..123519	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
BB344_RS26250	123516..123950	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
BB344_RS26255	124019..125983	-	1965	WP_001537566.1	ParB/RepB/Spo0J family partition protein	-
BB344_RS26260	126044..126277	-	234	WP_000006003.1	DUF905 family protein	-
BB344_RS26265	126335..126862	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
BB344_RS26275	127164..127601	+	438	WP_024185955.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / tet(A)	senB	1..151033	151033

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T64731 WP_001312861.1 NZ_CP016498:c122520-122362 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T64731 NZ_CP016498:c122520-122362 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT64731 NZ_CP016498:c122788-122564 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin