Detailed information of TA system TA064727

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 80077..80330
• Replicon: plasmid unnamed1
• Accession: NZ_CP016498
• Organism: Escherichia coli strain UPEC 26-1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: BB344_RS25980
• Protein ID: WP_001336447.1
• Coordinates: 80077..80226 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	80274..80330 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB344_RS25950	76102..76503	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
BB344_RS30415	76436..76693	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
BB344_RS25960	76786..77439	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
BB344_RS25965	78377..79234	-	858	WP_001537577.1	incFII family plasmid replication initiator RepA	-
BB344_RS25970	79227..79301	-	75	WP_001365705.1	RepA leader peptide Tap	-
BB344_RS25975	79535..79792	-	258	WP_000084404.1	replication regulatory protein RepA	-
BB344_RS25980	80077..80226	-	150	WP_001336447.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	80274..80330	+	57	NuclAT_1	-	Antitoxin
-	80274..80330	+	57	NuclAT_1	-	Antitoxin
-	80274..80330	+	57	NuclAT_1	-	Antitoxin
-	80274..80330	+	57	NuclAT_1	-	Antitoxin
BB344_RS25985	80425..80862	-	438	WP_000872609.1	hypothetical protein	-
BB344_RS25990	81015..81398	-	384	WP_001109264.1	hypothetical protein	-
BB344_RS25995	81501..81962	-	462	WP_000760080.1	thermonuclease family protein	-
BB344_RS26000	82715..82918	-	204	WP_001336517.1	hypothetical protein	-
BB344_RS26005	83070..83627	-	558	WP_000139329.1	fertility inhibition protein FinO	-
BB344_RS26010	83682..84428	-	747	WP_000205709.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / tet(A)	senB	1..151033	151033

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T64727 WP_001336447.1 NZ_CP016498:c80226-80077 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T64727 NZ_CP016498:c80226-80077 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT64727 NZ_CP016498:80274-80330 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin