Detailed information of TA system TA064726

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5015004..5015731
• Replicon: chromosome
• Accession: NZ_CP016497
• Organism: Escherichia coli strain UPEC 26-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: BB344_RS24595
• Protein ID: WP_000547555.1
• Coordinates: 5015004..5015315 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BB344_RS24600
• Protein ID: WP_065726115.1
• Coordinates: 5015312..5015731 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB344_RS24565	5010145..5011854	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
BB344_RS24570	5011864..5012406	+	543	WP_000493797.1	formate hydrogenlyase subunit HycF	-
BB344_RS24575	5012406..5013173	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
BB344_RS24580	5013170..5013580	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
BB344_RS24585	5013573..5014043	+	471	WP_000132956.1	hydrogenase maturation peptidase HycI	-
BB344_RS24590	5014086..5014841	+	756	WP_001305268.1	hypothetical protein	-
BB344_RS24595	5015004..5015315	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
BB344_RS24600	5015312..5015731	+	420	WP_065726115.1	helix-turn-helix domain-containing protein	Antitoxin
BB344_RS24605	5015823..5016251	-	429	WP_000536064.1	hypothetical protein	-
BB344_RS24610	5016636..5017163	+	528	WP_001078777.1	electron transport protein HydN	-
BB344_RS24615	5017316..5019568	+	2253	WP_001107659.1	carbamoyltransferase HypF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T64726 WP_000547555.1 NZ_CP016497:5015004-5015315 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T64726 NZ_CP016497:5015004-5015315 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCTTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATCACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15440.49 Da
• Isoelectric Point: 4.4596

>AT64726 WP_065726115.1 NZ_CP016497:5015312-5015731 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGIKPEWFL

Nucleotide

• Length: 420 bp

>AT64726 NZ_CP016497:5015312-5015731 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCAGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATACCCCACATTAAGGCACTTTCAGCCCGATTTGGCATTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: V0YLE2