Detailed information of TA system TA064721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3583305..3583907
• Replicon: chromosome
• Accession: NZ_CP016497
• Organism: Escherichia coli strain UPEC 26-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: BB344_RS17580
• Protein ID: WP_000897302.1
• Coordinates: 3583596..3583907 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BB344_RS17575
• Protein ID: WP_000356397.1
• Coordinates: 3583305..3583595 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB344_RS17545	3579378..3580280	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
BB344_RS17550	3580277..3580912	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
BB344_RS17555	3580909..3581838	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
BB344_RS17560	3582054..3582272	-	219	WP_042042063.1	ribbon-helix-helix domain-containing protein	-
BB344_RS17565	3582668..3582946	-	279	WP_001296612.1	hypothetical protein	-
BB344_RS17575	3583305..3583595	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
BB344_RS17580	3583596..3583907	-	312	WP_000897302.1	hypothetical protein	Toxin
BB344_RS17585	3584137..3585045	+	909	WP_001305059.1	alpha/beta hydrolase	-
BB344_RS17590	3585109..3586050	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
BB344_RS17595	3586095..3586532	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
BB344_RS17600	3586529..3587401	-	873	WP_000920762.1	virulence factor BrkB family protein	-
BB344_RS17605	3587395..3587994	-	600	WP_001443191.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T64721 WP_000897302.1 NZ_CP016497:c3583907-3583596 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T64721 NZ_CP016497:c3583907-3583596 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT64721 WP_000356397.1 NZ_CP016497:c3583595-3583305 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT64721 NZ_CP016497:c3583595-3583305 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416