Detailed information of TA system TA064715

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-ohsC/SymE(toxin)
• Location: 2964231..2964643
• Replicon: chromosome
• Accession: NZ_CP016497
• Organism: Escherichia coli strain UPEC 26-1

Toxin (Protein)

• Gene name: symE
• Uniprot ID: Q8FA88
• Locus tag: BB344_RS14425
• Protein ID: WP_000132630.1
• Coordinates: 2964302..2964643 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	ohsC
Locus tag	-
Coordinates	2964231..2964307 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB344_RS14415	2960843..2962312	+	1470	WP_001315223.1	type I restriction-modification system subunit M	-
BB344_RS14420	2962312..2964081	+	1770	WP_000110076.1	restriction endonuclease subunit S	-
-	2964231..2964307	-	77	NuclAT_6	-	Antitoxin
-	2964231..2964307	-	77	NuclAT_6	-	Antitoxin
-	2964231..2964307	-	77	NuclAT_6	-	Antitoxin
-	2964231..2964307	-	77	NuclAT_6	-	Antitoxin
-	2964231..2964307	-	77	NuclAT_7	-	Antitoxin
-	2964231..2964307	-	77	NuclAT_7	-	Antitoxin
-	2964231..2964307	-	77	NuclAT_7	-	Antitoxin
-	2964231..2964307	-	77	NuclAT_7	-	Antitoxin
BB344_RS14425	2964302..2964643	+	342	WP_000132630.1	endoribonuclease SymE	Toxin
BB344_RS14430	2964690..2966783	-	2094	WP_000648234.1	DUF262 domain-containing protein	-
BB344_RS29190	2966881..2966961	-	81	WP_020233658.1	hypothetical protein	-
BB344_RS14435	2967189..2968109	-	921	WP_000181195.1	Rpn family recombination-promoting nuclease/putative transposase	-
BB344_RS14440	2968294..2969574	+	1281	WP_001304526.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12294.13 Da
• Isoelectric Point: 8.4982

>T64715 WP_000132630.1 NZ_CP016497:2964302-2964643 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T64715 NZ_CP016497:2964302-2964643 [Escherichia coli]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT64715 NZ_CP016497:c2964307-2964231 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0E0Y5T0

Antitoxin