Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1258544..1259106 Replicon chromosome
Accession NZ_CP016497
Organism Escherichia coli strain UPEC 26-1

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag BB344_RS05965 Protein ID WP_000605675.1
Coordinates 1258544..1258822 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag BB344_RS05970 Protein ID WP_000781370.1
Coordinates 1258822..1259106 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB344_RS05940 1254130..1254561 - 432 WP_000152307.1 peroxiredoxin OsmC -
BB344_RS05945 1254906..1255121 + 216 WP_000495766.1 biofilm-dependent modulation protein -
BB344_RS05950 1255223..1255360 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
BB344_RS05955 1255517..1257214 + 1698 WP_000433460.1 malate dehydrogenase -
BB344_RS05960 1257348..1258358 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
BB344_RS05965 1258544..1258822 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BB344_RS05970 1258822..1259106 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
BB344_RS05975 1259335..1259988 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
BB344_RS05980 1259981..1260865 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
BB344_RS05985 1260878..1263925 - 3048 WP_011076399.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T64710 WP_000605675.1 NZ_CP016497:1258544-1258822 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T64710 NZ_CP016497:1258544-1258822 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGTAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT64710 WP_000781370.1 NZ_CP016497:1258822-1259106 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT64710 NZ_CP016497:1258822-1259106 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCTACGGCAAGTCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTGTCTGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References