Detailed information of TA system TA064698

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hokW-rdlD/-
• Location: 761012..761237
• Replicon: chromosome
• Accession: NZ_CP016497
• Organism: Escherichia coli strain UPEC 26-1

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: BB344_RS03305
• Protein ID: WP_000813254.1
• Coordinates: 761082..761237 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	rdlD
Locus tag	-
Coordinates	761012..761070 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB344_RS03255	756274..756426	-	153	WP_000379609.1	DUF1391 family protein	-
BB344_RS03265	756698..756985	-	288	WP_000936799.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BB344_RS03270	756985..757176	-	192	WP_000100899.1	hypothetical protein	-
BB344_RS03275	757204..757605	-	402	WP_001329851.1	helix-turn-helix domain-containing protein	-
BB344_RS03280	757715..757987	+	273	WP_000887447.1	helix-turn-helix domain-containing protein	-
BB344_RS03285	757971..758396	+	426	WP_000693918.1	Rha family transcriptional regulator	-
BB344_RS03290	758468..759538	+	1071	WP_001262355.1	hypothetical protein	-
BB344_RS03295	759579..760004	+	426	WP_001151152.1	DUF977 family protein	-
BB344_RS03300	760179..760844	+	666	WP_000150294.1	epoxyqueuosine reductase QueH	-
-	761012..761070	-	59	-	-	Antitoxin
BB344_RS03305	761082..761237	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
BB344_RS28805	761405..761683	+	279	WP_024193993.1	hypothetical protein	-
BB344_RS03315	761685..762734	+	1050	WP_001265292.1	DUF968 domain-containing protein	-
BB344_RS03320	762747..763121	+	375	WP_001217427.1	RusA family crossover junction endodeoxyribonuclease	-
BB344_RS03325	763118..763939	+	822	WP_001235237.1	antitermination protein	-
BB344_RS03330	764185..764898	+	714	WP_000342820.1	hypothetical protein	-
BB344_RS03355	765623..765838	+	216	WP_000839572.1	class II holin family protein	-
BB344_RS03360	765843..766157	+	315	WP_000193273.1	YdfR family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T64698 WP_000813254.1 NZ_CP016497:761082-761237 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T64698 NZ_CP016497:761082-761237 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT64698 NZ_CP016497:c761070-761012 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin