Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1673414..1674026 Replicon chromosome
Accession NZ_CP016464
Organism Bosea sp. RAC05

Toxin (Protein)


Gene name higB Uniprot ID A0A1B3NF27
Locus tag BSY19_RS11370 Protein ID WP_069054269.1
Coordinates 1673730..1674026 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1B3NQE0
Locus tag BSY19_RS11365 Protein ID WP_069054268.1
Coordinates 1673414..1673722 (-) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSY19_RS11345 1670317..1670937 - 621 WP_069054264.1 DsbA family protein -
BSY19_RS11350 1671045..1671542 - 498 WP_069054265.1 DUF721 domain-containing protein -
BSY19_RS11355 1671586..1672074 - 489 WP_069054266.1 hypothetical protein -
BSY19_RS11360 1672268..1673389 + 1122 WP_069054267.1 PA0069 family radical SAM protein -
BSY19_RS11365 1673414..1673722 - 309 WP_069054268.1 HigA family addiction module antidote protein Antitoxin
BSY19_RS11370 1673730..1674026 - 297 WP_069054269.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BSY19_RS11375 1674138..1674749 + 612 WP_069054270.1 ribonuclease HII -
BSY19_RS11380 1675132..1676211 + 1080 WP_171905224.1 site-specific DNA-methyltransferase -
BSY19_RS11385 1676214..1676510 - 297 WP_069054271.1 helix-turn-helix domain-containing protein -
BSY19_RS11390 1676507..1676854 - 348 WP_069054272.1 type II toxin-antitoxin system RelE/ParE family toxin -
BSY19_RS11395 1676904..1677956 - 1053 WP_069054273.1 A/G-specific adenine glycosylase -
BSY19_RS11400 1678004..1678402 - 399 WP_069054274.1 GFA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-95)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(5-82)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11660.23 Da        Isoelectric Point: 6.9900

>T64666 WP_069054269.1 NZ_CP016464:c1674026-1673730 [Bosea sp. RAC05]
MIVSFRDEWLRAFFVEDVHAKHIPPDLETRLFRKLQMIDDAVTDSDLRVPPSNHFEKLRGALDGLHSIRVNQQWRLIFAW
DGRRGEARGLYLDDHSYR

Download         Length: 297 bp

>T64666 NZ_CP016464:c1674026-1673730 [Bosea sp. RAC05]
ATGATCGTCAGCTTTCGGGATGAGTGGCTGCGCGCCTTCTTCGTCGAAGATGTCCACGCGAAACACATTCCGCCCGATCT
CGAGACGCGCCTGTTCCGGAAATTGCAGATGATCGACGACGCCGTCACCGATTCTGATCTGCGCGTGCCCCCGAGCAATC
ATTTCGAGAAGCTGCGCGGGGCGCTGGACGGCCTGCACAGCATCCGCGTCAACCAGCAGTGGCGGCTGATCTTCGCCTGG
GACGGCCGTCGCGGCGAAGCCCGCGGCCTCTATCTCGACGACCACAGCTATCGATAG

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11621.58 Da        Isoelectric Point: 10.9057

>AT64666 WP_069054268.1 NZ_CP016464:c1673722-1673414 [Bosea sp. RAC05]
MLTTKRKPATVGEILVEEFMRPLGLTQIRLAQAMGVPRKHVNELCRDRRGLTAATALILARVFGNSAEFWLNVQRRTDLW
ETLHSPEEMARIERARPLEVAA

Download         Length: 309 bp

>AT64666 NZ_CP016464:c1673722-1673414 [Bosea sp. RAC05]
ATGCTGACGACGAAACGCAAGCCCGCCACGGTCGGTGAAATCCTGGTCGAGGAGTTCATGCGGCCGCTGGGGCTGACGCA
GATCCGGCTGGCCCAGGCGATGGGCGTGCCCCGCAAGCATGTCAACGAACTCTGCCGGGATCGTCGAGGTCTCACGGCTG
CGACGGCGCTGATCCTGGCACGCGTCTTCGGCAACTCCGCCGAGTTCTGGCTCAATGTGCAGCGACGGACCGATCTCTGG
GAGACACTGCATTCGCCCGAGGAGATGGCGCGCATCGAGCGGGCGCGGCCATTGGAAGTCGCGGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B3NF27

Antitoxin

Source ID Structure
AlphaFold DB A0A1B3NQE0

References