Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2209700..2210296 Replicon chromosome
Accession NZ_CP016450
Organism Sinorhizobium sp. RAC02

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag BSY16_RS10625 Protein ID WP_069059628.1
Coordinates 2210015..2210296 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID -
Locus tag BSY16_RS10620 Protein ID WP_069059627.1
Coordinates 2209700..2209999 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSY16_RS10600 2204838..2206109 + 1272 WP_069059623.1 phosphoribosylamine--glycine ligase -
BSY16_RS10605 2206214..2206690 + 477 WP_069059624.1 hypothetical protein -
BSY16_RS10610 2206791..2207285 - 495 WP_069059625.1 DUF523 domain-containing protein -
BSY16_RS10615 2207299..2209608 - 2310 WP_069059626.1 glycine--tRNA ligase subunit beta -
BSY16_RS10620 2209700..2209999 - 300 WP_069059627.1 HigA family addiction module antidote protein Antitoxin
BSY16_RS10625 2210015..2210296 - 282 WP_069059628.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BSY16_RS10630 2210361..2212298 - 1938 WP_069059629.1 DUF2207 domain-containing protein -
BSY16_RS10635 2212313..2212861 - 549 WP_069059630.1 LemA family protein -
BSY16_RS10640 2213085..2213282 + 198 WP_069059631.1 hypothetical protein -
BSY16_RS10645 2213390..2214280 + 891 WP_069059632.1 alpha/beta hydrolase -
BSY16_RS10650 2214288..2215250 - 963 WP_150129930.1 glycine--tRNA ligase subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(23-69)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10625.25 Da        Isoelectric Point: 9.1751

>T64648 WP_069059628.1 NZ_CP016450:c2210296-2210015 [Sinorhizobium sp. RAC02]
MIRAFRGKLVEMVADSSVKKGFPPELVRRAQQLLTVLHAAVPIEDLRSPPGNRLEKLAGDREGQHSIRINRQWHICFRWT
AAGPEDVEIVDYH

Download         Length: 282 bp

>T64648 NZ_CP016450:c2210296-2210015 [Sinorhizobium sp. RAC02]
ATGATCAGGGCATTCCGGGGCAAGCTTGTCGAAATGGTCGCCGATAGTTCGGTGAAGAAGGGATTTCCTCCTGAACTCGT
TCGACGCGCCCAGCAATTGCTCACTGTGCTGCACGCCGCGGTACCGATTGAAGATCTTCGGTCACCACCCGGTAACCGGC
TGGAAAAACTGGCGGGCGATCGGGAAGGGCAACATTCGATCCGCATCAACAGGCAATGGCATATCTGTTTTCGCTGGACG
GCGGCCGGGCCTGAGGATGTTGAGATTGTCGATTATCATTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10895.73 Da        Isoelectric Point: 9.6923

>AT64648 WP_069059627.1 NZ_CP016450:c2209999-2209700 [Sinorhizobium sp. RAC02]
MVSPKLPPIHPGEILNDLYLAPLEMSAGALAGKLNVPRTRVERIVSGRVGITSDTALRLAKFFGTTPEFWMNLQTAYELK
IEAERKKADIARIPVHDAA

Download         Length: 300 bp

>AT64648 NZ_CP016450:c2209999-2209700 [Sinorhizobium sp. RAC02]
ATGGTCTCGCCAAAGCTGCCGCCGATCCACCCGGGGGAAATCCTGAACGATCTCTATCTTGCCCCGCTGGAGATGAGCGC
GGGCGCGCTTGCAGGTAAATTGAACGTGCCGCGGACGCGAGTCGAGCGCATCGTTTCCGGCCGTGTCGGCATCACCTCCG
ATACGGCGCTTCGCCTCGCCAAGTTTTTCGGCACGACGCCCGAATTCTGGATGAATTTACAGACGGCTTATGAGTTGAAG
ATCGAAGCGGAGCGCAAGAAGGCCGATATTGCACGCATTCCCGTTCATGACGCGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References