Detailed information of TA system TA064627

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 621590..622226
• Replicon: chromosome
• Accession: NZ_CP016433
• Organism: Rhizobium sp. S41

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A098RNJ2
• Locus tag: BA939_RS17655
• Protein ID: WP_006697304.1
• Coordinates: 621945..622226 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: BA939_RS17650
• Protein ID: WP_004444714.1
• Coordinates: 621590..621889 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BA939_RS17625	616776..617504	-	729	WP_037092566.1	ABC transporter ATP-binding protein	-
BA939_RS17630	617501..618409	-	909	WP_037092567.1	branched-chain amino acid ABC transporter permease	-
BA939_RS17635	618406..619278	-	873	WP_037092568.1	branched-chain amino acid ABC transporter permease	-
BA939_RS17640	619349..620563	-	1215	WP_037092569.1	amino acid ABC transporter substrate-binding protein	-
BA939_RS17645	620696..621570	+	875	Protein_619	helix-turn-helix transcriptional regulator	-
BA939_RS17650	621590..621889	-	300	WP_004444714.1	HigA family addiction module antidote protein	Antitoxin
BA939_RS17655	621945..622226	-	282	WP_006697304.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BA939_RS17660	622301..623110	-	810	WP_037092570.1	ABC transporter ATP-binding protein	-
BA939_RS17665	623107..624075	-	969	WP_037092571.1	iron chelate uptake ABC transporter family permease subunit	-
BA939_RS17670	624078..625121	-	1044	WP_037092572.1	iron ABC transporter permease	-
BA939_RS17675	625118..626125	-	1008	WP_037092598.1	iron-siderophore ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (19-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10536.95 Da
• Isoelectric Point: 9.0298

>T64627 WP_006697304.1 NZ_CP016433:c622226-621945 [Rhizobium sp. S41]
MIRSFKNKLAASIDDGSVKKGFPSDMLRRAQQLLTVLDAATTVEDPRSPPGNRLEKLSGDREGQYSIRINKQWRICFVWT
QAGPESVEITDYH

Nucleotide

• Length: 282 bp

>T64627 NZ_CP016433:c622226-621945 [Rhizobium sp. S41]
ATGATCCGTTCTTTCAAAAACAAGCTGGCTGCTTCGATTGACGACGGATCTGTGAAGAAGGGATTTCCCTCCGATATGCT
ACGGCGCGCCCAGCAACTGCTCACGGTCCTGGATGCGGCGACTACGGTAGAAGACCCTCGCTCACCGCCCGGCAATCGTT
TGGAAAAGCTGTCCGGCGATCGTGAGGGCCAATATTCGATCCGGATCAACAAGCAGTGGCGCATCTGCTTCGTCTGGACG
CAAGCAGGGCCGGAAAGCGTGGAAATAACCGACTATCACTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11060.86 Da
• Isoelectric Point: 8.5148

>AT64627 WP_004444714.1 NZ_CP016433:c621889-621590 [Rhizobium sp. S41]
MTISKSPPIHPGEILRELYLEPLGMSAYALARHLNVPRTRIERIAGEKLGITADTALRLAKFFKTTPEYWLNLQNAFDIK
AAAARLETDLANIKELEAA

Nucleotide

• Length: 300 bp

>AT64627 NZ_CP016433:c621889-621590 [Rhizobium sp. S41]
ATGACAATCAGTAAATCGCCACCGATCCATCCGGGCGAAATCCTGCGGGAACTCTATCTTGAGCCACTGGGGATGTCGGC
ATACGCGCTCGCAAGGCACCTCAATGTTCCGCGCACCCGCATAGAACGGATTGCAGGAGAGAAACTTGGTATCACCGCGG
ACACCGCCTTGCGTTTGGCAAAGTTTTTCAAGACCACCCCGGAATATTGGCTGAACTTGCAGAACGCTTTTGACATTAAG
GCGGCTGCAGCGAGGCTGGAAACGGACCTTGCAAATATCAAGGAACTGGAAGCAGCGTAG

Structures

Toxin

• AlphaFold DB: A0A098RNJ2