Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_31
Location 4612567..4613168 Replicon chromosome
Accession NZ_CP016428
Organism Bradyrhizobium icense strain LMTR 13

Toxin (Protein)


Gene name higB Uniprot ID A0A1B1USL4
Locus tag LMTR13_RS21755 Protein ID WP_065732873.1
Coordinates 4612845..4613168 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1B1UI34
Locus tag LMTR13_RS21750 Protein ID WP_065729613.1
Coordinates 4612567..4612848 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMTR13_RS21730 4608235..4609139 - 905 Protein_4379 DMT family transporter -
LMTR13_RS21735 4609320..4609727 + 408 WP_065732872.1 response regulator -
LMTR13_RS21740 4609743..4611977 + 2235 WP_065729611.1 CHASE3 domain-containing protein -
LMTR13_RS21745 4612076..4612414 + 339 WP_065729612.1 HNH endonuclease -
LMTR13_RS21750 4612567..4612848 - 282 WP_065729613.1 helix-turn-helix transcriptional regulator Antitoxin
LMTR13_RS21755 4612845..4613168 - 324 WP_065732873.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LMTR13_RS21760 4613321..4614385 - 1065 WP_065729614.1 hypothetical protein -
LMTR13_RS21765 4614837..4616153 + 1317 WP_083219126.1 pilus assembly protein -
LMTR13_RS21770 4616165..4616668 + 504 WP_083219127.1 pilus assembly protein -
LMTR13_RS21775 4616680..4617042 + 363 WP_083219481.1 pilus assembly protein -
LMTR13_RS21780 4617115..4618011 - 897 WP_065729616.1 DMT family transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(15-104)

Antitoxin

Cro/C1-type helix-turn-helix domain

(35-83)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12055.15 Da        Isoelectric Point: 10.7842

>T64618 WP_065732873.1 NZ_CP016428:c4613168-4612845 [Bradyrhizobium icense]
VAWRVEILNELVAAEIASLPSDMQARFLRLAERISLAGLESLSEPHVKHLEGKLWELRLTGRDGIARALYVTAIGRRVVI
VRAFVKKTQKTPRSEIELALQRAKEVT

Download         Length: 324 bp

>T64618 NZ_CP016428:c4613168-4612845 [Bradyrhizobium icense]
GTGGCCTGGCGGGTAGAAATCCTCAACGAATTGGTCGCTGCGGAAATTGCTTCCCTTCCGAGTGACATGCAGGCGCGATT
TCTGCGACTAGCAGAGCGCATCAGCTTGGCCGGGCTAGAAAGCCTGAGCGAGCCGCACGTCAAACATCTCGAAGGGAAGC
TATGGGAGTTGCGGCTGACAGGTCGTGACGGAATTGCTCGCGCGCTTTATGTCACGGCGATTGGCCGAAGGGTGGTCATA
GTTCGCGCATTTGTAAAGAAGACTCAAAAGACTCCACGCTCGGAGATCGAGCTCGCGCTGCAGCGAGCAAAGGAGGTCAC
ATGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10226.93 Da        Isoelectric Point: 11.0624

>AT64618 WP_065729613.1 NZ_CP016428:c4612848-4612567 [Bradyrhizobium icense]
MTIPFEKIKARLLANPKVKAEYEALAPEFEIAAELLRARLRAGLSQAELAARMRTSQSTIARLESGQTLPSTKTLLRYAQ
ATGSKFHVRLSAA

Download         Length: 282 bp

>AT64618 NZ_CP016428:c4612848-4612567 [Bradyrhizobium icense]
ATGACAATTCCGTTTGAGAAGATCAAAGCTCGTCTCTTGGCCAATCCGAAGGTGAAGGCGGAGTACGAGGCACTCGCTCC
AGAGTTCGAAATTGCCGCCGAGTTGCTTAGGGCTCGCCTGCGCGCAGGTCTCTCGCAAGCCGAACTGGCTGCTCGGATGA
GGACCAGCCAATCCACGATCGCACGACTTGAAAGCGGTCAAACGTTACCGAGCACGAAGACACTGCTGCGCTATGCCCAA
GCAACCGGTAGTAAGTTTCACGTGCGGCTGTCGGCCGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B1USL4

Antitoxin

Source ID Structure
AlphaFold DB A0A1B1UI34

References