Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2831074..2831576 Replicon chromosome
Accession NZ_CP016404
Organism Escherichia coli strain 210221272

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag BBP24_RS14070 Protein ID WP_000767829.1
Coordinates 2831074..2831328 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag BBP24_RS14075 Protein ID WP_001259255.1
Coordinates 2831325..2831576 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BBP24_RS14045 2826089..2826316 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
BBP24_RS14050 2826330..2827388 - 1059 WP_023568741.1 transport protein YeeE -
BBP24_RS14055 2827567..2828925 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
BBP24_RS25660 2828915..2828977 - 63 WP_010723108.1 membrane protein YoeI -
BBP24_RS14060 2829192..2830121 - 930 WP_000803351.1 LysR family transcriptional regulator -
BBP24_RS14065 2830167..2830991 - 825 WP_000754763.1 SDR family oxidoreductase -
BBP24_RS14070 2831074..2831328 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
BBP24_RS14075 2831325..2831576 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
BBP24_RS24615 2831858..2831908 + 51 WP_001364200.1 his operon leader peptide -
BBP24_RS14080 2832054..2832953 + 900 WP_000131776.1 ATP phosphoribosyltransferase -
BBP24_RS14085 2832959..2834263 + 1305 WP_001317939.1 histidinol dehydrogenase -
BBP24_RS14090 2834260..2835330 + 1071 WP_001410397.1 histidinol-phosphate transaminase -
BBP24_RS14095 2835330..2836397 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T64588 WP_000767829.1 NZ_CP016404:c2831328-2831074 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T64588 NZ_CP016404:c2831328-2831074 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT64588 WP_001259255.1 NZ_CP016404:c2831576-2831325 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT64588 NZ_CP016404:c2831576-2831325 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References